Gene Editing

Synthetic Biology

Created by Steven Burgess in Research - Synthetic Biology as public


  1. Plant gene editing through de novo induction of meristems

    Posted by Steven Burgess in Research - Synthetic Biology


  2. WheatCRISPR: a web-based guide RNA design tool for CRISPR/Cas9-mediated genome editing in wheat | BMC Plant Biology

    Posted by Steven Burgess in Research - Synthetic Biology


  3. Engineered Cas9 variant tools expand targeting scope of genome and base editing in rice

    Posted by Steven Burgess in Research - Synthetic Biology


  4. CRISPR/Cas9-mediated gene targeting in Arabidopsis using sequential transformation

    Posted by Steven Burgess in Research - Synthetic Biology


  5. A modular cloning toolkit for genome editing in plants

    Posted by Steven Burgess in Research - Synthetic Biology


  6. CRISPR-TSKO facilitates efficient cell type-, tissue-, or organ-specific mutagenesis in Arabidopsis

    Posted by Steven Burgess in Research - Synthetic Biology


  7. An expanded toolkit for precision genome engineering

    Posted by Steven Burgess in Research - Synthetic Biology


  8. Identification of transgene-free CRISPR edited plants of rice and tomato by monitoring DsRED fluorescence in dry seeds

    Posted by Steven Burgess in Research - Synthetic Biology


  9. ​Single transcript unit CRISPR 2.0 systems for robust Cas9 and Cas12a mediated plant genome editing

    Posted by Steven Burgess in Research - Synthetic Biology


  10. xCas9 expands the scope of genome editing with reduced efficiency in rice

    Posted by Steven Burgess in Research - Synthetic Biology


  11. Developing a highly efficient and wildly adaptive CRISPR-SaCas9 toolset for plant genome editing

    Posted by Steven Burgess in Research - Synthetic Biology


  12. Zinc finger nuclease‐mediated precision genome editing of an endogenous gene in hexaploid bread wheat (Triticum aestivum) using a DNA repair template

    Posted by Steven Burgess in Research - Synthetic Biology


  13. CRISPR/Cas9 editing of endogenous banana streak virus in the B genome of Musa spp. overcomes a major challenge in banana breeding

    Posted by Steven Burgess in Research - Synthetic Biology


  14. CRISPR‐PLANT v2: An online resource for highly specific guide RNA spacers based on improved off‐target analysis

    Posted by Steven Burgess in Research - Synthetic Biology


  15. Enhanced genome editing in rice using single transcript unit CRISPR‐LbCpf1 systems

    Posted by Steven Burgess in Research - Synthetic Biology


  16. Efficient C-to-T base editing in plants using a fusion of nCas9 and human APOBEC3A

    Posted by Steven Burgess in Research - Synthetic Biology


  17. Expanding the base editing scope in rice by using Cas9 variants  

    Posted by Steven Burgess in Research - Synthetic Biology


  18. Efficient Mitochondrial Genome Editing by CRISPR/Cas9

    Posted by Suphia Rafique in Research - Synthetic Biology


  19. Zinc finger nuclease‐mediated targeting of multiple transgenes to an endogenous soybean genomic locus via non‐homologous end joining

    Posted by Steven Burgess in Research - Synthetic Biology


  20. Development of germ-line-specific CRISPR-Cas9 systems to improve the production of heritable gene modifications in Arabidopsis

    Posted by Suphia Rafique in Research - Synthetic Biology


  21. Heat-shock inducible CRISPR/Cas9 system generates heritable mutations in rice  

    Posted by Steven Burgess in Research - Synthetic Biology


  22. A CRISPR-based approach for targeted DNA demethylation

    Posted by Suphia Rafique in Research - Synthetic Biology


  23. Both CRISPR/Cas-based nucleases and nickases can be used efficiently for genome engineering in Arabidopsis thaliana 

    Posted by Suphia Rafique in Research - Synthetic Biology


  24. Optimization of T-DNA architecture for Cas9-mediated mutagenesis in Arabidopsis

    Posted by Steven Burgess in Research - Synthetic Biology


  25. Application of protoplast technology to CRISPR/Cas9 mutagenesis: from single‐cell mutation detection to mutant plant regeneration

    Posted by Steven Burgess in Research - Synthetic Biology


  26. Comparison of efficiency and specificity of CRISPR-associated (Cas) nucleases in plants: An expanded toolkit for precision genome engineering

    Posted by Steven Burgess in Research - Synthetic Biology


  27. Visualization analysis of CRISPR/Cas9 gene editing technology studies

    Posted by Suphia Rafique in Research - Synthetic Biology


  28. De novo domestication of wild tomato using genome editing

    Posted by Steven Burgess in Research - Synthetic Biology


  29. Generation of inheritable and “transgene clean” targeted genome-modified rice in later generations using the CRISPR/Cas9 system

    Posted by Suphia Rafique in Research - Synthetic Biology


  30. In vivo assembly of DNA-fragments in the moss, Physcomitrella patens

    Posted by Konstantinos Vavitsas in Research - Synthetic Biology


  31. A versatile and robust Agrobacterium‐based gene stacking system generates high‐quality transgenic Arabidopsis plants

    Posted by Konstantinos Vavitsas in Research - Synthetic Biology


  32. Multiplexed Gene Editing and Protein Overexpression Using a Tobacco mosaic virus Viral Vector

    Posted by Steven Burgess in Research - Synthetic Biology


  33. A CRISPR/Cas9 Toolbox for Multiplexed Plant Genome Editing and Transcriptional Regulation

    Posted by Steven Burgess in Research - Synthetic Biology


  34. A CRISPR–Cpf1 system for efficient genome editing and transcriptional repression in plants

    Posted by Steven Burgess in Research - Synthetic Biology


  35. Improved Base Editor for Efficiently Inducing Genetic Variations in Rice with CRISPR/Cas9-Guided Hyperactive hAID Mutant

    Posted by Steven Burgess in Research - Synthetic Biology


  36. Precise A·T to G·C Base Editing in the Rice Genome

    Posted by Steven Burgess in Research - Synthetic Biology


  37. Engineering Quantitative Trait Variation for Crop Improvement by Genome Editing

    Posted by Steven Burgess in Research - Synthetic Biology