Gene Editing

Synthetic Biology

Created by Steven Burgess in Synthetic Biology as public


  1. CRISPR-TSKO facilitates efficient cell type-, tissue-, or organ-specific mutagenesis in Arabidopsis

    Posted by Steven Burgess in Synthetic Biology


  2. An expanded toolkit for precision genome engineering

    Posted by Steven Burgess in Synthetic Biology


  3. Identification of transgene-free CRISPR edited plants of rice and tomato by monitoring DsRED fluorescence in dry seeds

    Posted by Steven Burgess in Synthetic Biology


  4. ​Single transcript unit CRISPR 2.0 systems for robust Cas9 and Cas12a mediated plant genome editing

    Posted by Steven Burgess in Synthetic Biology


  5. xCas9 expands the scope of genome editing with reduced efficiency in rice

    Posted by Steven Burgess in Synthetic Biology


  6. Developing a highly efficient and wildly adaptive CRISPR-SaCas9 toolset for plant genome editing

    Posted by Steven Burgess in Synthetic Biology


  7. Zinc finger nuclease‐mediated precision genome editing of an endogenous gene in hexaploid bread wheat (Triticum aestivum) using a DNA repair template

    Posted by Steven Burgess in Synthetic Biology


  8. CRISPR/Cas9 editing of endogenous banana streak virus in the B genome of Musa spp. overcomes a major challenge in banana breeding

    Posted by Steven Burgess in Synthetic Biology


  9. CRISPR‐PLANT v2: An online resource for highly specific guide RNA spacers based on improved off‐target analysis

    Posted by Steven Burgess in Synthetic Biology


  10. Enhanced genome editing in rice using single transcript unit CRISPR‐LbCpf1 systems

    Posted by Steven Burgess in Synthetic Biology


  11. Efficient C-to-T base editing in plants using a fusion of nCas9 and human APOBEC3A

    Posted by Steven Burgess in Synthetic Biology


  12. Expanding the base editing scope in rice by using Cas9 variants  

    Posted by Steven Burgess in Synthetic Biology


  13. Efficient Mitochondrial Genome Editing by CRISPR/Cas9

    Posted by Suphia Rafique in Synthetic Biology


  14. Zinc finger nuclease‐mediated targeting of multiple transgenes to an endogenous soybean genomic locus via non‐homologous end joining

    Posted by Steven Burgess in Synthetic Biology


  15. Development of germ-line-specific CRISPR-Cas9 systems to improve the production of heritable gene modifications in Arabidopsis

    Posted by Suphia Rafique in Synthetic Biology


  16. Heat-shock inducible CRISPR/Cas9 system generates heritable mutations in rice  

    Posted by Steven Burgess in Synthetic Biology


  17. A CRISPR-based approach for targeted DNA demethylation

    Posted by Suphia Rafique in Synthetic Biology


  18. Both CRISPR/Cas-based nucleases and nickases can be used efficiently for genome engineering in Arabidopsis thaliana 

    Posted by Suphia Rafique in Synthetic Biology


  19. Optimization of T-DNA architecture for Cas9-mediated mutagenesis in Arabidopsis

    Posted by Steven Burgess in Synthetic Biology


  20. Application of protoplast technology to CRISPR/Cas9 mutagenesis: from single‐cell mutation detection to mutant plant regeneration

    Posted by Steven Burgess in Synthetic Biology


  21. Comparison of efficiency and specificity of CRISPR-associated (Cas) nucleases in plants: An expanded toolkit for precision genome engineering

    Posted by Steven Burgess in Synthetic Biology


  22. Visualization analysis of CRISPR/Cas9 gene editing technology studies

    Posted by Suphia Rafique in Synthetic Biology


  23. De novo domestication of wild tomato using genome editing

    Posted by Steven Burgess in Synthetic Biology


  24. Generation of inheritable and “transgene clean” targeted genome-modified rice in later generations using the CRISPR/Cas9 system

    Posted by Suphia Rafique in Synthetic Biology


  25. In vivo assembly of DNA-fragments in the moss, Physcomitrella patens

    Posted by Konstantinos Vavitsas in Synthetic Biology


  26. A versatile and robust Agrobacterium‐based gene stacking system generates high‐quality transgenic Arabidopsis plants

    Posted by Konstantinos Vavitsas in Synthetic Biology


  27. Multiplexed Gene Editing and Protein Overexpression Using a Tobacco mosaic virus Viral Vector

    Posted by Steven Burgess in Synthetic Biology


  28. A CRISPR/Cas9 Toolbox for Multiplexed Plant Genome Editing and Transcriptional Regulation

    Posted by Steven Burgess in Synthetic Biology


  29. A CRISPR–Cpf1 system for efficient genome editing and transcriptional repression in plants

    Posted by Steven Burgess in Synthetic Biology


  30. Improved Base Editor for Efficiently Inducing Genetic Variations in Rice with CRISPR/Cas9-Guided Hyperactive hAID Mutant

    Posted by Steven Burgess in Synthetic Biology


  31. Precise A·T to G·C Base Editing in the Rice Genome

    Posted by Steven Burgess in Synthetic Biology


  32. Engineering Quantitative Trait Variation for Crop Improvement by Genome Editing

    Posted by Steven Burgess in Synthetic Biology