Gene Editing

Synthetic Biology

Created by Steven Burgess in Synthetic Biology as public

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  1. Zinc finger nuclease‐mediated precision genome editing of an endogenous gene in hexaploid bread wheat (Triticum aestivum) using a DNA repair template

    Posted by Steven Burgess in Synthetic Biology


  2. CRISPR‐PLANT v2: An online resource for highly specific guide RNA spacers based on improved off‐target analysis

    Posted by Steven Burgess in Synthetic Biology


  3. Enhanced genome editing in rice using single transcript unit CRISPR‐LbCpf1 systems

    Posted by Steven Burgess in Synthetic Biology


  4. Efficient C-to-T base editing in plants using a fusion of nCas9 and human APOBEC3A

    Posted by Steven Burgess in Synthetic Biology


  5. Expanding the base editing scope in rice by using Cas9 variants  

    Posted by Steven Burgess in Synthetic Biology


  6. Efficient Mitochondrial Genome Editing by CRISPR/Cas9

    Posted by Suphia Rafique in Synthetic Biology


  7. Zinc finger nuclease‐mediated targeting of multiple transgenes to an endogenous soybean genomic locus via non‐homologous end joining

    Posted by Steven Burgess in Synthetic Biology


  8. Development of germ-line-specific CRISPR-Cas9 systems to improve the production of heritable gene modifications in Arabidopsis

    Posted by Suphia Rafique in Synthetic Biology


  9. Heat-shock inducible CRISPR/Cas9 system generates heritable mutations in rice  

    Posted by Steven Burgess in Synthetic Biology


  10. A CRISPR-based approach for targeted DNA demethylation

    Posted by Suphia Rafique in Synthetic Biology


  11. Both CRISPR/Cas-based nucleases and nickases can be used efficiently for genome engineering in Arabidopsis thaliana 

    Posted by Suphia Rafique in Synthetic Biology


  12. Optimization of T-DNA architecture for Cas9-mediated mutagenesis in Arabidopsis

    Posted by Steven Burgess in Synthetic Biology


  13. Application of protoplast technology to CRISPR/Cas9 mutagenesis: from single‐cell mutation detection to mutant plant regeneration

    Posted by Steven Burgess in Synthetic Biology


  14. Comparison of efficiency and specificity of CRISPR-associated (Cas) nucleases in plants: An expanded toolkit for precision genome engineering

    Posted by Steven Burgess in Synthetic Biology


  15. Visualization analysis of CRISPR/Cas9 gene editing technology studies

    Posted by Suphia Rafique in Synthetic Biology


  16. De novo domestication of wild tomato using genome editing

    Posted by Steven Burgess in Synthetic Biology


  17. Generation of inheritable and “transgene clean” targeted genome-modified rice in later generations using the CRISPR/Cas9 system

    Posted by Suphia Rafique in Synthetic Biology


  18. In vivo assembly of DNA-fragments in the moss, Physcomitrella patens

    Posted by Konstantinos Vavitsas in Synthetic Biology


  19. A versatile and robust Agrobacterium‐based gene stacking system generates high‐quality transgenic Arabidopsis plants

    Posted by Konstantinos Vavitsas in Synthetic Biology


  20. Multiplexed Gene Editing and Protein Overexpression Using a Tobacco mosaic virus Viral Vector

    Posted by Steven Burgess in Synthetic Biology


  21. A CRISPR/Cas9 Toolbox for Multiplexed Plant Genome Editing and Transcriptional Regulation

    Posted by Steven Burgess in Synthetic Biology


  22. A CRISPR–Cpf1 system for efficient genome editing and transcriptional repression in plants

    Posted by Steven Burgess in Synthetic Biology


  23. Improved Base Editor for Efficiently Inducing Genetic Variations in Rice with CRISPR/Cas9-Guided Hyperactive hAID Mutant

    Posted by Steven Burgess in Synthetic Biology


  24. Precise A·T to G·C Base Editing in the Rice Genome

    Posted by Steven Burgess in Synthetic Biology


  25. Engineering Quantitative Trait Variation for Crop Improvement by Genome Editing

    Posted by Steven Burgess in Synthetic Biology