Abstract

A variety of sequence‐specific nucleases (SSNs) have been successfully used for plant genome editing. Clustered regularly interspaced short palindromic repeat (CRISPR)‐CRISPR‐associated (Cas) systems, which were developed from microbial adaptive immune systems, are preferred SSN tools due to their simplicity and versatility. The majority of plant genome editing studies have exploited a CRISPR‐Cas9 system from Streptococcus pyogenes (SpCas9) to achieve site‐specific mutagenesis and perform fragment insertion or replacement in organisms ranging from algae to higher plants. A newly identified Cas endonuclease named Cpf1 is also considered a promising genome editing tool (Zetsche et al., 2015).

https://doi.org/10.1111/pbi.13028