- Exploiting Day- and Night-Time Metabolism of Synechocystis sp. PCC 6803 for Fitness-Coupled Fumarate Production around the Clock
- Optogenetic manipulation of stomatal kinetics improves carbon assimilation, water use, and growth
- An improved Escherichia coli screen for Rubisco identifies a protein–protein interface that can enhance CO2-fixation kinetics
- Plant RuBisCo assembly in E. coli with five chloroplast chaperones including BSD2
- Artificial photosynthetic cell producing energy for protein synthesis
- Genetic Engineering, Synthetic Biology and the Light Reactions of Photosynthesis
- Enzyme kinetics of tobacco Rubisco expressed in Escherichia coli varies depending on the small subunit composition
- A short history of RubisCO: the rise and fall (?) of Nature's predominant CO2 fixing enzyme
- Photorespiratory bypass boost crop growth and productivity in field
- Synthetic glycolate metabolism pathways stimulate crop growth and productivity in the field
- Engineering Strategies to Boost Crop Productivity by Cutting Respiratory Carbon Loss
- Improving the efficiency of photosynthetic carbon reactions
- Photosynthetic fuel for heterologous enzymes: the role of electron carrier proteins
- Light-driven chemical synthesis
- Genetic engineering, synthetic biology and the light reactions of photosynthesis
- Rational engineering of photosynthetic electron flux enhances light-powered cytochrome P450 activity
- Refactoring the Six-Gene Photosystem II Core in the Chloroplast of the Green Algae Chlamydomonas reinhardtii
- Photosynthetic artificial organelles sustain and control ATP-dependent reactions in a protocellular system
Engineering Strategies to Boost Crop Productivity by Cutting Respiratory Carbon Loss
- © 2019 American Society of Plant Biologists. All rights reserved.
Roughly half the carbon that crop plants fix by photosynthesis is subsequently lost by respiration. Nonessential respiratory activity leading to unnecessary CO2 release is unlikely to have been minimized by natural selection or crop breeding, and cutting this large loss could complement and rein¬force the currently dominant yield-enhancement strategy of increasing carbon fixation. Until now, however, respiratory carbon losses have generally been overlooked by metabolic engineering and synthetic biology be¬cause specific target genes have been elusive. We argue that recent advances are at last pinpointing individual enzyme and transporter genes that can be engineered to (i) slow unnecessary protein turn¬over, (ii) replace, relocate, or reschedule metabolic activities, (iii) suppress futile cycles, and (iv) make ion transport more efficient, all of which can reduce respiratory costs. We identify a set of engineering strategies to reduce respiratory carbon loss that are now feasible, and model how implementing these strategies singly or in tandem could lead to substantial gains in crop productivity.
- Received October 1, 2018.
- Accepted January 9, 2019.
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