Synbio cloning systems

Created by Steven Burgess in Synthetic Biology as public


  1. Universal Loop assembly (uLoop): open, efficient, and species-agnostic DNA fabrication

    Posted by Steven Burgess in Synthetic Biology


  2. MoChlo: A versatile modular cloning toolbox for chloroplast biotechnology

    Posted by Steven Burgess in Synthetic Biology


  3. Golden Mutagenesis: An efficient multi-site saturation mutagenesis approach by Golden Gate cloning with automated primer design 

    Posted by Steven Burgess in Synthetic Biology


  4. CyanoGate: A Golden Gate modular cloning suite for engineering cyanobacteria based on the plant MoClo syntax

    Posted by Steven Burgess in Synthetic Biology


  5. Birth of a photosynthetic chassis: a MoClo toolkit enabling synthetic biology in the microalga Chlamydomonas reinhardtii

    Posted by Konstantinos Vavitsas in Synthetic Biology


  6. Loop Assembly: a simple and open system for recursive fabrication of DNA circuits

    Posted by Steven Burgess in Synthetic Biology


  7. AQUA Cloning: A Versatile and Simple Enzyme-Free Cloning Approach

    Posted by Steven Burgess in Synthetic Biology


  8. GoldenBraid 2.0: A Comprehensive DNA Assembly Framework for Plant Synthetic Biology

    Posted by Steven Burgess in Synthetic Biology


  9. A Golden Gate Modular Cloning Toolbox for Plants

    Posted by Steven Burgess in Open Forum


  10. A versatile and robust Agrobacterium‐based gene stacking system generates high‐quality transgenic Arabidopsis plants

    Posted by Konstantinos Vavitsas in Synthetic Biology


  11. A robust gene-stacking method utilizing yeast assembly for plant synthetic biology

    Posted by Steven Burgess in Synthetic Biology


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