Molecular Tools

Created by Steven Burgess in Synthetic Biology as public


  1. Engineered PPR proteins as inducible switches to activate the expression of chloroplast transgenes

    Posted by Steven Burgess in Synthetic Biology


  2. Characterising standard genetic parts and establishing common principles for engineering legume and cereal roots  

    Posted by Steven Burgess in Synthetic Biology


  3. Strong gene activation in plants with genome‐wide specificity using a new orthogonal CRISPR/Cas9‐based Programmable Transcriptional Activator

    Posted by Steven Burgess in Synthetic Biology


  4. A promiscuous split intein with expanded protein engineering applications

    Posted by Steven Burgess in Synthetic Biology


  5. Phenotypes on demand via switchable target protein degradation in multicellular organisms

    Posted by Steven Burgess in Synthetic Biology


  6. Recent Advances and Current Challenges in Synthetic Biology of the Plastid Genetic System and Metabolism

    Posted by Steven Burgess in Synthetic Biology


  7. Tandem fluorescent protein timers for non-invasive relative protein lifetime measurement in plants

    Posted by Steven Burgess in Synthetic Biology


  8. Spatiotemporal Gene Repression System in the Heterocyst-Forming Multicellular Cyanobacterium Anabaena sp. PCC 7120

    Posted by Steven Burgess in Synthetic Biology


  9. Switching toxic protein function in life cells

    Posted by Steven Burgess in Synthetic Biology


  10. Site-specific manipulation of Arabidopsis loci using CRISPR-Cas9 SunTag systems

    Posted by Steven Burgess in Synthetic Biology


  11. Harnessed viruses in the age of metagenomics and synthetic biology: An update on infectious clone assembly and biotechnologies of plant viruses

    Posted by Steven Burgess in Synthetic Biology


  12. Endoribonuclease-Based Two-Component Repressor Systems for Tight Gene Expression Control in Plants

    Posted by Steven Burgess in Synthetic Biology


  13. Split-TALE: A TALE-based two-component system for synthetic biology applications in planta

    Posted by Steven Burgess in Synthetic Biology


  14. Plant Synthetic Biology: Quantifying the Known Unknowns and Discovering the Unknown Unknowns 

    Posted by Steven Burgess in Synthetic Biology


  15. Quantitative characterization of genetic parts and circuits for plant synthetic biology

    Posted by Steven Burgess in Synthetic Biology


  16. Chimeric 3’ flanking regions strongly enhance gene expression in plants

    Posted by Steven Burgess in Synthetic Biology


  17. A potent Cas9-derived gene activator for plant and mammalian cells

    Posted by Steven Burgess in Synthetic Biology


  18. MarpoDB: An Open Registry for Marchantia Polymorpha Genetic Parts

    Posted by Steven Burgess in Synthetic Biology


  19. A Cyan Fluorescent Reporter Expressed from the Chloroplast Genome of Marchantia polymorpha

    Posted by Steven Burgess in Synthetic Biology


  20. Agrochemical control of plant water use using engineered abscisic acid receptors

    Posted by Steven Burgess in Synthetic Biology


  21. Synthetic hormone-responsive transcription factors can monitor and re-program plant development

    Posted by Steven Burgess in Synthetic Biology


  22. Multiplexed Gene Editing and Protein Overexpression Using a Tobacco mosaic virus Viral Vector

    Posted by Steven Burgess in Synthetic Biology


  23. Stabilization and translation of synthetic operon‐derived mRNAs in chloroplasts by sequences representing PPR protein‐binding sites

    Posted by Steven Burgess in Synthetic Biology


  24. Synthetic hormone-responsive transcription factors can monitor and re-program plant development

    Posted by Steven Burgess in Synthetic Biology


  25. Synthetic MicroProteins: Versatile Tools for Posttranslational Regulation of Target Proteins

    Posted by Steven Burgess in Synthetic Biology