My overall research interests are focused on both plant development and abiotic stress effects, with a specifically interest in how the plant hormone cytokinin is involved in these processes. My lab uses molecular, genetic, and physiological approaches to study a set of transcription factors known as Cytokinin Response Factors (CRFs), which are regulated by both cytokinin and stress. A major goal of my research is a better understanding of how cytokinin and stress interact through CRFs in both Arabidopsis and tomato to control essential plant processes and development. My teaching interests parallel my research interests and are focused on plant biology, development, and plant hormones.
Research Areas: Abiotic Interactions, Genetics, Growth Regulation, Signals and Responses
Keshishian EA, Hallmark HT, Ramaraj T, Plačková L, Sundararajan A, Schilkey F, Novák O, Rashotte AM (2018) Salt and oxidative stresses uniquely regulate tomato cytokinin levels and transcriptomic response. Plant Direct 2:1–13.Striberny B, Melton AE, Schwacke R, Krause K, Fischer K, Goertzen LR, Rashotte AM (2017) Cytokinin Response Factor 5 has transcriptional activity governed by its C-terminal domain. Plant Signaling & Behavior 12: e1276684.
Zwack PJ, De Clercq I, Howton TC, Hallmark HT, Hurny A, Keshishian EA, Parish AM, Benkova E, Mukhtar MS, Van Breusegem F, Rashotte AM (2016) Cytokinin Response Factor 6 Represses Cytokinin-Associated Genes during Oxidative Stress. Plant Physiology. 172: 1249–1258.
Zwack PJ, Compton MA, Adams CI, Rashotte AM (2016) Cytokinin Response Factor 4 (CRF4) is induced by cold and involved in freezing tolerance. Plant Cell Reports. 35: 573–584.
Li R, Rashotte AM, Singh NK, Lawrence KS, Weaver DB, Locy RD (2015) Transcriptome Analysis of Cotton (Gossypium hirsutum L.) Genotypes That Are Susceptible, Resistant, and Hypersensitive to Reniform Nematode (Rotylenchulus reniformis). PLoS ONE. 10: e0143261.
Keshishian EA and Rashotte AM (2015) Plant Cytokinin Signalling. Essays in Biochemistry. 58: 13-27.
Hu H, Rashotte AM, Singh NK, Weaver DB, Goertzen LR, Singh SR, Locy RD (2015) The complexity of posttranscriptional small RNA regulatory networks revealed by in silico analysis of Gossypium arboreum L. leaf, flower and boll small regulatory RNAs. PLoS ONE 10: e0127468.
Zwack PJ and Rashotte AM (2015) Interactions between Cytokinin Signalling and Abiotic Stress Responses. Journal of Experimental Botany. 66: 4863-4871.
Li R, Rashotte AM, Singh NK, Weaver DB, Lawrence KS, Locy RD (2015) Integrated signaling networks in plant responses to sedentary endoparasitic nematodes: a perspective. Plant Cell Reports. 34: 5-22.
Wang Z, Rashotte AM, Dane F (2014) Citrullus colocynthis NAC transcription factors CcNAC1 and CcNAC2 are involved in light and auxin signaling. Plant Cell Reports. 33: 1673-1686.
Shi X, Gupta S, Rashotte AM (2014) Characterization of Two Tomato AP2/ERF genes, SlCRF1 and SlCRF2 in Hormone and Stress Responses. Plant Cell Reports 33: 35–45.
Gupta S and Rashotte AM (2014) Expression patterns of SlCRF3 and SlCRF5 in response to cytokinin and abiotic stress conditions in tomato (Solanum lycopersicum). Journal of Plant Physiology 171: 349-358.
Wang Z, Rashotte AM, Moss AG, Dane F (2014) Two NAC transcription factors from Citrullus colocynthis, CcNAC1, CcNAC2 implicated in multiple stress responses. Acta Physiologiae Plantarum 36: 621-634.
Zwack PJ and Rashotte AM (2013) Cytokinin Inhibition of Leaf Senescence. Plant Signaling & Behavior 8: e24737.
Zwack PJ, Robinson BR, Risley MG, Rashotte AM (2013) Cytokinin Response Factor 6 Negatively Regulates Leaf Senescence and is Induced in Response to Cytokinin and Numerous Abiotic Stresses. Plant & Cell Physiology 54: 971-981.
Shi X, Gupta S, Lindquist IE, Cameron CT, Mudge J, Rashotte AM (2013) Transcriptome analysis of cytokinin response in tomato leaves. PLoS ONE 8: e55090.
Gupta S, Shi X, Lindquist IE, Devitt NP, Mudge J, Rashotte AM (2013) Transcriptome profiling of cytokinin and auxin regulation in tomato root. Journal of Experimental Botany 64: 695-704.
Heyl A, Brault M, Frugier F, Kuderová A, Lindner A-C, Motyka V, Rashotte AM, von Schwartzenberg K, Vankova R, Schaller E. (2013) Nomenclature for members of the two-component signaling pathway of plants. Plant Physiology 161: 1063-1065.
Jung YJ, Lee IH, Nou IS, Lee KD, Rashotte AM, Kang KK. (2013) BrRZFP1 a Brassica rapa C3HC4-type RING zinc finger protein involved in cold, salt, and dehydration stress. Plant Biology 15: 274-283.
Zwack PJ, Shi X, Robinson BR, Gupta S, Gerken DM, Compton MA, Goertzen LR, Rashotte AM (2012) Vascular expression and C-terminal sequence divergence of Cytokinin Response Factors in flowering plants. Plant & Cell Physiology 53: 1683-1695.
Gupta S and Rashotte AM (2012) Down-stream components of cytokinin signaling and the role of cytokinin throughout the plant. Plant Cell Reports 31:801–812.
Shi X and Rashotte AM (2012) Advances in upstream players of cytokinin phosphorelay: receptors and histidine phosphotransfer proteins. Plant Cell Reports 31:789–799.
Shi X, Gupta S, Rashotte AM (2012) Solanum lycopersicum Cytokinin Response Factors (SlCRFs) genes: characterization of CRF domain containing ERF genes in tomato. Journal of Experimental Botany 63: 973–982.
Cutcliffe JW, Hellmann E, Heyl A, Rashotte AM (2011) CRFs form protein-protein interactions among each other and with members of the cytokinin-signaling pathway in Arabidopsis via the CRF domain. Journal of Experimental Botany 62:4995-5002.
Sang Y, Locy RD, Goertzen LR, Rashotte AM, Si Y, Kang K, Singh NK (2011) Expression, in vivo localization and phylogenetic analysis of pyridoxine 5’-phosphate oxidase in Arabidopsis thaliana. Plant Physiology and Biochemistry 49:88-95.
Yuan K, Rashotte AM, Wysocka-Diller J (2011) ABA and GA signaling pathways interact and regulate seed germination and seedling development under salt stress. Acta Physiologiae Plantarum. 33:261–271.
Rashotte AM and Goertzen LR (2010) The CRF domain defines Cytokinin Response Factor proteins in plants. BMC Plant Biology 10:74.
Si Y, Dane F, Rashotte A, Kang K, and Singh NK (2010) Cloning and expression analysis of Ccrboh gene encoding respiratory burst oxidase in Citrullus colocynthis and grafting onto Citrullus lantus (Watermelon). Journal of Experimental Botany 61:1635-1642.
Rashotte AM, Mason MM, Hutchison CE, Ferreira FJ, Schaller GE, Kieber JJ (2006) A subset of Arabidopsis AP2 transcription factors mediate cytokinin responses in concert with a two-component pathway: PNAS 103:11081-11085.
Rashotte AM, Chae HS, Maxwell BB, Kieber JJ (2005) The interaction of cytokinin with other signals: Physiologia Plantarum 123:184-194.
Poupart J, Rashotte AM, Muday GK, Waddell CS (2005) The rib1 mutant of Arabidopsis has alterations in Indole-3-Butyric Acid transport, hypocotyl elongation, and root architecture: Plant Physiology 139:1460-1471.
Goodwin SM, Rashotte AM, Rahman M, Feldmann KA, Jenks MA (2005) Wax constituents on the inflorescence stems of double eceriferum mutants in Arabidopsis reveal complex gene interactions. Phytochemistry 66:771-780.
Rashotte AM, Jenks MA, Ross AS, Feldmann KA (2004) Novel eceriferum mutants in Arabidopsis thaliana. Planta 219:5-13.
Rashotte AM, Carson SDB, To JPC, Kieber JJ (2003) Expression profiling of cytokinin action in Arabidopsis: Plant Physiology 132:1998-2011.
Rashotte AM, Poupart J, Waddell CS, Muday GK (2003) Transport of the two natural auxins, IBA and IAA, in Arabidopsis: Plant Physiology 133:761-772.
Wyatt SE, Rashotte AM, Shipp MJ, Robertson D, Muday GK (2002) Mutations in the gravity persistence signal loci in Arabidopsis disrupt the perception and/or signal transduction of gravitropic stimuli: Plant Physiology 130:1426-1435.
Long JC, Zhao W, Rashotte AM, Muday GK, Huber SC (2002) Gravity stimulated changes in auxin and invertase gene expression in maize pulvinal cells: Plant Physiology 128:591-602.
Rashotte AM, Delong A, Muday GK (2001) Genetic and chemical reductions in protein phosphatase activity alter auxin transport, gravity response and lateral root growth. Plant Cell 13:1683-1697.
Brown D, Rashotte AM, Murphy AS, Normanly J, Tague BW, Peer WA, Taiz L, Muday GK (2001) Flavonoids act as negative regulators of auxin transport in vivo in Arabidopsis. Plant Physiology 126:524-535.
Rashotte AM, Jenks MA, Feldmann KA (2001) Cuticular waxes on eceriferum mutants of Arabidopsis thaliana. Phytochemistry 57:115-123.
Rashotte AM, Brady SR, Reed RC, Ante SJ, Muday GK (2000) Basipetal auxin transport is required for gravitropism in roots of Arabidopsis. Plant Physiology 122:481-490.
Rashotte AM and Feldmann KA (1998) Correlations between epicuticular wax structures and chemical composition in Arabidopsis thaliana. International Journal of Plant Sciences 159:773-779.
Rashotte AM, Jenks MA, Nguyen TD, Feldmann KA (1997) Epicuticular wax variation in ecotypes of Arabidopsis thaliana. Phytochemistry 45:251-255.
Jenks MA, Rashotte AM, Tuttle HA, Feldmann KA (1996) Mutants in Arabidopsis thaliana altered in epicuticular wax and leaf morphology. Plant Physiology 110:377-38.
Destination STEM - Auburn University, Summer Science Institute (SSI) - Auburn University