Bio

Research Areas:
Biochemistry, Evolution, Genetics, Genomics, Metabolism

Publications

1.  MacCoss, M., F.K. Ryu, R.S. White and R.L. Last. 1980. A new synthetic use of nucleoside N-oxides. J. Org. Chem. 45:788-794.

2.  Last, R.L., J.B. Stavenhagen and J.L. Woolford. 1984. Isolation and characterization of RNA2, RNA3, and RNA11 genes of yeast. Mol. Cell. Biol. 4:2396-2405.

3.  Last, R.L. and J.L. Woolford. 1986. Identification and nuclear localization of yeast pre-messenger RNA processing components: RNA2 and RNA3 proteins. J. Cell. Biol. 103:2103-2112.

4.  Last, R.L., J. Maddock and J.L. Woolford. 1987. Evidence for related functions of the RNA genes of yeast. Genetics 117:619-631.

5.  Last, R.L. and G.R. Fink. 1988. Tryptophan-requiring mutants of the plant Arabidopsis thaliana. Science 240:305-310.

6.  Berlyn, M.B., R.L. Last and G.R. Fink. 1989. A gene encoding the tryptophan synthase ß subunit of Arabidopsis thaliana. Proc. Natl. Acad. Sci. U.S.A. 86:4604-4608.

7.  Last, R.L., P.H. Bissinger, D.J. Mahoney, E.R. Radwanski and G.R. Fink. 1991. Tryptophan mutants in Arabidopsis: the consequences of duplicated tryptophan synthase ß genes. Plant Cell 3:345-358.

8.  Rose, A.B., A.L. Casselman and R.L. Last. 1992. A phosphoribosylanthranilate transferase gene is defective in blue fluorescent Arabidopsis thaliana tryptophan mutants. Plant Physiol. 100:582-592.

9.  Last, R.L., A.J. Barczak, A.L. Casselman, J. Li, K.D. Pruitt, E.R. Radwanski and A.B. Rose. 1992. The molecular genetics of tryptophan biosynthesis in Arabidopsis thaliana. In: Biosynthesis and Molecular Regulation of Amino Acids in Plants. B.K. Singh, H.E. Flores and J.C. Shannon, eds. Current Topics in Plant Physiology: an American Society of Plant Physiologists Series, vol. 7, pp. 28-36.

10.   Li, J., T.-M. Ou-Lee, R. Raba, R.G. Amundson and R.L. Last. 1993. Arabidopsis flavonoid mutants are hypersensitive to UV-B irradiation. Plant Cell 5:171-179.

11.   Pruitt, K.D. and R.L. Last. 1993. Expression patterns of duplicate tryptophan synthase ß genes in Arabidopsis thaliana. Plant Physiol. 102:1019-1026.

12.   Niyogi, K.K., R.L. Last, G.R. Fink and B. Keith. 1993. Suppressors of trp1 fluorescence identify a new Arabidopsis gene, TRP4, encoding the anthranilate synthase beta subunit. Plant Cell 5:1011-1027.

13.   Tsuji, J., M. Zook, S.C. Somerville, R.L. Last and R. Hammerschmidt.  1993. Evidence that tryptophan is not a direct biosynthetic intermediate of camalexin in Arabidopsis thaliana. Physiol. and Molec. Plant Path. 43:221-229.

14.   Last, R.L. 1993. The genetics of nitrogen assimilation and amino acid biosynthesis in flowering plants: progress and prospects. In: International Review of Cytology. K.W. Jeon, J. Jarvik and M. Friedlander, eds. Academic Press, NY, vol. 143:297-330.

15.   Raikhel, N.V. and R.L. Last. 1993. The wide world of plant molecular biology. Plant Cell 5:823-830 (Meeting Review).

16.   Last, R.L., A.J. Barczak, M. Ho, J. Li, K.D. Pruitt, E.R. Radwanski and A.B. Rose. 1994. Genetic dissection of tryptophan biosynthesis in Arabidopsis thaliana. In: Plant Molecular Biology 3. G. Coruzzi and P. Puigdomènech, eds. NATO ASI Series vol. H81. Springer Verlag, Heidelberg, pp. 151-158.

17.   Rose, A.B. and R.L. Last. 1994. Molecular genetics of amino acid, nucleotide, and vitamin biosynthesis. In: Arabidopsis. C.R. Somerville and E.M. Meyerowitz, eds. Cold Spring Harbor Press, NY, pp. 835-879.

18.   Barczak, A.J., J. Zhao, K.D. Pruitt and R.L. Last. 1995. 5-Fluoroindole resistance identifies tryptophan synthase beta subunit mutants in Arabidopsis thaliana. Genetics 140:303-313.

19.   Conklin, P.L. and R.L. Last. 1995. Differential accumulation of antioxidant mRNAs in Arabidopsis thaliana exposed to ozone. Plant Physiol. 109:203-212.

20.   Landry, L.G., C.C.S. Chapple and R.L. Last. 1995. Arabidopsis mutants lacking phenolic sunscreens exhibit enhanced ultraviolet-B injury and oxidative damage. Plant Physiol. 109:1159-1166.

21.   Li, J., J. Zhao, A.B. Rose, R. Schmidt and R.L. Last. 1995. Arabidopsis phosphoribosylanthranilate isomerase: molecular genetic analysis of triplicate tryptophan pathway genes. Plant Cell 7:447-461.

22.   Li, J., S. Chen, L. Zhu and R.L. Last. 1995. Isolation of cDNAs encoding the tryptophan pathway enzyme indole-3-glycerol phosphate synthase from Arabidopsis thaliana. Plant Physiol. 108:877-878.

23.   Radwanski, E.R., J. Zhao and R.L. Last. 1995. Arabidopsis thaliana tryptophan synthase alpha: gene cloning, expression, and subunit interaction. Mol. Gen. Genet. 248:657-667.

24.   Zhao, J. and R.L. Last. 1995. Immunological characterization and chloroplast localization of the tryptophan biosynthetic enzymes of the flowering plant Arabidopsis thaliana. J. Biol. Chem. 270:6081-6087.

25.   Radwanski, E.R. and R.L. Last. 1995. Tryptophan biosynthesis and metabolism: Biochemical and molecular genetics. Plant Cell 7:921-934.

26.   Li, J. and R.L. Last. 1996. The Arabidopsis thaliana trp5 mutant has a feedback-resistant anthranilate synthase and elevated soluble tryptophan. Plant Physiol. 110:51-59.

27.   Conklin, P.L., E.H. Williams and R.L. Last. 1996. Environmental stress sensitivity of an ascorbic acid-deficient Arabidopsis mutant. Proc. Natl. Acad. Sci. U.S.A. 93:9970-9974.

28.   Radwanski, E.R., A.J. Barczak and R.L. Last. 1996. Characterization of tryptophan synthase alpha subunit mutants of Arabidopsis thaliana. Mol. Gen. Genet. 253:353-361.

29.   Zhao, J. and R.L. Last.  1996. Coordinate regulation of tryptophan biosynthetic pathway and indolic phytoalexin accumulation in Arabidopsis. Plant Cell 8:2235-2244.

30.   Last, R.L. and L.G. Landry. 1996. Arabidopsis thaliana as a model for studies of UV-B adaptation. In: Regulation of Plant Growth and Development by Light. W.R. Briggs, R.L. Heath and E.M. Tobin, eds.  Current Topics in Plant Physiology: an American Society of Plant Physiologists Series, vol. 17:74-79.

31.   Rose, A.B, J. Li and R.L. Last. 1997. An allelic series of blue fluorescent trp1 mutants of Arabidopsis thaliana. Genetics 145:197-205.

32.   Landry, L.G., A.E. Stapleton, J. Lim, P. Hoffman, J.B. Hays, V. Walbot and R.L. Last. 1997. An Arabidopsis photolyase mutant is hypersensitive to ultraviolet-B radiation. Proc. Natl. Acad. Sci. U.S.A. 94:328-332.

33.   Rose, A.B. and R.L. Last. 1997. Introns act post-transcriptionally to increase expression of the Arabidopsis thaliana tryptophan pathway gene PAT1. Plant J. 11: 455-464.

34.   Ahmad, M., J.A. Jarillo, L.J. Klimczak, L.G. Landry, T. Peng, R.L. Last and A.R. Cashmore. 1997. An enzyme similar to animal type II photolyases mediates photoreactivation in Arabidopsis. Plant Cell 9:199-207.

35.   Conklin, P.L., J.E. Pallanca, R.L. Last and N. Smirnoff. 1997. L-Ascorbic acid metabolism in the ascorbate-deficient Arabidopsis mutant vtc1. Plant Physiol. 115:1277-1285.

36.   Zhao, J., C.C. Williams and R.L. Last. 1998. Induction of Arabidopsis tryptophan pathway enzymes and camalexin by amino acid starvation, oxidative stress, and an abiotic elicitor. Plant Cell 10: 359-370.

37.   Kim, B.C., D.J. Tennessen, and R.L. Last. 1998. UV-B induced photomorphogenesis in Arabidopsis thaliana, Plant J. 15:667-674.

38.   Kliebenstein, D.J., R.-A. Monde, and R.L. Last. 1998. Superoxide dismutase in Arabidopsis: An eclectic enzyme family with disparate regulation and protein localization. Plant Physiol. 118: 637-650.

39.   Denby, K.J. and R.L. Last. 1998. Genetic approaches to understanding the regulation of tryptophan biosynthesis. In: Cellular Integration of Signaling Pathways in Plant Development. F. Lo Schiavo, R.L. Last, G. Morelli and N. Raikhel, eds. NATO ASI Series vol. H104. Springer Verlag, Heidelberg, pp. 159-170.

40.   Conklin, P.L., S.R. Norris, G.L. Wheeler, E. H. Williams, N. Smirnoff, and R.L. Last. 1999. Genetic evidence for the role of GDP-mannose in plant ascorbic acid (Vitamin C) biosynthesis. Proc. Natl. Acad. Sci. U.S.A. 96:4198-4203.

41.   Kliebenstein, D.J., R.A. Dietrich, A.C. Martin, R.L. Last, and J.L. Dangl. 1999. LSD1 regulates salicylic acid induction of copper zinc superoxide dismutase in Arabidopsis thaliana. Molec. Plant Microbe Interact. 12:1022-1026.

42.   Denby, K.J. and R.L. Last. 1999. Diverse Regulatory Mechanisms of Amino Acid Biosynthesis In Plants. In: Genetic Engineering, Volume 21, pp. 173-189.

43.   Rose, A.B., R.L. Last. 1999. Methods and compositions for enhancing the expression of genes in plants. US Patent 5,861,277.

44.   Conklin, P.L., S.A. Saracco, S.R. Norris, and R.L. Last. 2000. Identification of ascorbic acid-deficient Arabidopsis thaliana mutants. Genetics 154: 847-856.

45.   Coruzzi, G.M. and R.L. Last. 2000. Amino Acids. In: Biochemistry and Molecular Biology of Plants. R.B. Buchanan, W. Gruissem and R. Jones, eds. Am. Soc. Plant Physiology Press. pp. 358-410.

46.   Lukowitz, W., T.C. Nickle, D.W. Meinke, R.L. Last, P.L. Conklin, C.R. Somerville. 2001. Arabidopsis cyt1 mutants are deficient in a mannose-1-phosphate guanylyltransferase and point to a requirement of N-linked glycosylation for cellulose biosynthesis. Proc. Natl. Acad. Sci. U.S.A. 98:2262-2267.

47.   Last, R.L. and L. Wilmitzer. 2001. Physiology and metabolism: editorial overview. In: Current Opinions in Plant Biology. 4: 179-180.

48.   Jander, G, S.R. Norris, S. Rounsley, I. Levin, and R.L. Last. 2002. Arabidopsis map-based cloning in the post-genome era. Plant Physiol., 129: 440-450.

49.   Kliebenstein, D.J., J. E. Lim, L.G. Landry, R. L. Last. 2002. The Arabidopsis RCC1 homologue UVR8 mediates UV-B signal transduction and tolerance. Plant Physiol., 130: 234-243.

50.   Jander, G., S.R. Baerson, J.A. Hudak, K.A. Gonzalez, K.R. Gruys, and R.L. Last. 2003. Saturation mutagenesis in Arabidopsis to determine frequency of herbicide resistance. Plant Physiol., 131: 139-146.

51.   Last, R.L. 2003. Sandbox ethics in science: sharing of data and materials in plant biology. Plant Physiol., 132: 17-18 (Introductory article to Editor’s Choice series on Sharing of Biological Data and Materials).

52.   Sweetlove, L.J., R.L. Last, and A.R. Fernie 2003. Predictive Metabolic Engineering: a Goal for Systems Biology, Plant Physiol., 132: 420-425.

53.   Van Eenennaam, A.L., K. Lincoln, T.P. Durrett, H.E. Valentin, C.K. Shewmaker, G.M. Thorne, J. Jiang, S.R. Baszis , C.K. Levering, E.D. Aasen, M. Hao, J.C. Stein, S.R. Norris, and R.L. Last 2003. Engineering improved vitamin E quality: from Arabidopsis mutant to soy oil. Plant Cell, 15: 3007-3019

54.   Kim, J.H., T.P. Durrett, R.L. Last, and G. Jander. 2004. Characterization of the Arabidopsis TU8 glucosinolate mutation, an allele of TERMINAL FLOWER2. Plant Mol. Biol. 54: 671-682

55.   Jander, G., S.R. Norris, V. Joshi, A. Rugg, M. Fraga, S. Yu, L. Li, and R.L. Last. 2004. Application of a high-throughput HPLC-MS/MS assay to Arabidopsis mutant screening; evidence that threonine aldolase plays a role in seed nutritional quality.  Plant J. 39:465-475.

56.   Denby, K.J., L.J.M. Jason, S.L. Murray, and R.L. Last. 2005. ups1, an Arabidopsis thaliana camalexin accumulation mutant defective in multiple defence signalling pathways. Plant J. 41:673-84.

57.   Valentin, H.E., K. Lincoln, F. Moshiri, P.K. Jensen, Q. Qi, T.V. Venkatesh, B. Karunanandaa, S.R. Baszis, S.R. Norris, B. Savidge, K.J. Gruys, R.L. Last. 2006. The Arabidopsis vte5-1 Mutant Reveals a Critical Role for Phytol Kinase in Seed Tocopherol Biosynthesis. Plant Cell 18: 212-224.

58.   DellaPenna, D. and R.L. Last. 2006. Progress in the dissection and manipulation of plant vitamin E biosynthesis. Physiol. Plant. 126:356-368.

59.   Fiehn O, Sumner LW, Rhee SY, Ward J, Dickerson J, Lange BM, Lane G, Roessner U, Last R, Nikolau B 2007. Minimum reporting standards for plant biology context information in metabolomic studies. Metabolomics 3, 195-201 

60.   Xin, X., A. Mandaokar, J. Chen, R.L. Last and J. Browse. 2007. Arabidopsis ESK1 encodes a novel regulator of freezing tolerance. Plant J.49: 786-799.

61.   Last, R.L., A.D. Jones and Y. Shachar-Hill. 2007. Towards the Plant Metabolome and Beyond. Nature Rev. Molec. Cell. Biol. 8, 167-174.

62.   Kliebenstein, D.J., J.C. D’Auria, A.S. Behere, J.H. Kim, K.L. Gunderson, J.N. Breen, G., J. Gershenzon, R.L. Last and G. Jander. 2007. Characterization of seed-specific benzoyloxyglucosinolate mutations in Arabidopsis thaliana. Plant J. 51, 1062–1076.

63.   Gu, L., A.D. Jones and R.L. Last. 2007. LC-MS/MS assay for protein amino acids and metabolically related compounds for large-scale screening of metabolic phenotypes. Anal. Chem. 79, 8067-8075.

64.   Schilmiller, A.L., R.L. Last and E. Pichersky. 2008. Harnessing plant trichome biochemistry for the production of useful compounds. Plant J. 54:702-711.

65.   Lee, M., T. Toro-Ramos, T. Huang, M. Fraga, R.L. Last and G. Jander. 2008. Reduced activity of Arabidopsis thaliana HMT2, a methionine biosynthetic enzyme, increases seed methionine content. Plant J. 54:310-20.

66.   Lu, Y.  L.J. Savage, I. Ajjawi, K.M. Imre, D.W. Yoder, C. Benning, D. DellaPenna, J.B. Ohlrogge, K.W. Osteryoung, A.P. Weber, C.G. Wilkerson and R.L. Last. 2008. New connections across pathways and cellular processes: industrialized mutant screening reveals novel associations between diverse phenotypes in Arabidopsis. Plant Physiol., 146: 1482-1500.

67.   DellaPenna, D. and R.L. Last 2008. The expanding universe of genomics enabled plant biochemistry. Science. 320:479-481.

68.   Lu, Y. and Last R.L. (2008) Web-Based Arabidopsis Functional and Structural Genomics Resources. The Arabidopsis Book 6:e0118. doi:10.1199/tab.0118.

69.   Schilmiller*, A.L., I. Schauvinhold*, M. Larson, R. Xu, A.L. Charbonneau, A. Schmidt, R.L. Last and E. Pichersky 2009. Monoterpenes in the glandular trichomes of tomato are synthesized via a neryl diphosphate intermediate rather than geranyl diphosphate. Proc. Natl. Acad. Sci. U.S.A. 106:10865-70 doi: 10.1073/pnas.0904113106 (commentary by Bohlman and Gershenzon (2009) Proc. Natl. Acad. U.S.A. 106:10402-10403)

70.   Gao* J.,  I. Ajjawi*, A. Manoli, A. Sawin, C. Xu, J. Froehlich, R. L. Last and C. Benning 2009. FATTY ACID DESATURASE 4 of Arabidopsis encodes a protein distinct from classic fatty acid desaturases. Plant J. 60:832-839.

71.   Ajjawi, I., Y. Lu, L.J. Savage, S.M. Bell and R.L. Last. 2010. Large scale reverse genetics in Arabidopsis: Case studies from the Chloroplast 2010 Project. Plant Physiol.  152:529-540.

72.   Gu, L., A.D. Jones and R.L. Last. 2010. Metabolite profiling reveals broad metabolic phenotypes associated with a plant amino acid catabolism mutant. Plant J. 61:579-590.

73.   Rounsley, S.D. and R.L. Last. 2010. Shotguns and SNPs: how fast and cheap sequencing is revolutionizing plant biology. Plant J. 61: 922–927

74.   Schilmiller, A.L*., F. Shi*, J. Kim, A.L. Charbonneau, D. Holmes, A.D. Jones, R.L. Last. 2010. Mass spectrometry screening reveals widespread diversity in trichome specialized metabolites of tomato chromosomal substitution lines. Plant J. 62: 391–403.

75.   Schilmiller, A.L., D.P. Miner, M. Larson, E. Mc Dowell, D.R. Gang, C. Wilkerson, R.L. Last. 2010. Studies of a biochemical factory: Tomato trichome deep EST sequencing and proteomics. Plant Physiol. 153:1212-1223.

76.   Buell, C.R. and R.L. Last. 2010. Twenty-First Century Plant Biology: Impacts of the Arabidopsis Genome on Plant Biology and Agriculture. Plant Physiol. 154:497-500.

77.   Lu, Y., L.R. Savage, M. Larson, C. Wilkerson and R.L. Last. 2011. Chloroplast 2010: A database for large-scale phenotypic screening of Arabidopsis mutants. Plant Physiol. 155:1589-1600.

78.   Lu, Y., L.R. Savage and R.L. Last. 2011. Systematic Phenotypic Screening of Chloroplast Protein Mutants in Arabidopsis. Chloroplast Research in Arabidopsis: Methods and Protocols in Methods in Molecular Biology. R.P. Jarvis, ed. Humana Press, NY. 775:161-185.

79.   Lu, Y., D.A. Hall and R.L. Last. 2011. A small zinc finger thylakoid protein plays a role in maintenance of photosystem II. Plant Cell, 23: 1861-1875.

80.   Falara, V., T. Akhtar, T.H. Nguyen, E.A. Spyropoulou, P.M. Bleeker, I. Schauvinhold, Y. Matsuba, M.E. Bonini, A.L. Schilmiller, R.L. Last, R.C. Schuurink and E. Pichersky. 2011. The tomato (Solanum lycopersicum) terpene synthase gene family. Plant Physiol. 157:770-89. doi: 10.1104/pp.111.179648

81.   Ajjawi, I., A. Coku, Y. Yang, J.E. Froehlich, K.W. Osteryoung, C. Benning and R.L. Last. 2011. A J-like protein influences fatty acid composition of chloroplast lipids in Arabidopsis. PLoSONE 6(10): e25368. doi:10.1371/journal.pone.

0025368.

82.   Gu, L., A.D. Jones and R.L. Last. 2012. Rapid LC−MS/MS Profiling of protein amino acids and metabolically related compounds for large-scale assessment of metabolic phenotypes. Amino Acid Analysis Handbook in Methods in Molecular Biology. M.A. Alterman and P. Hunziker, eds. Humana Press, NY. 828:1-11.

83.   Schilmiller, A.L., E. Pichersky and R.L. Last. 2012. Taming the hydra of specialized metabolism: how systems biology and comparative approaches are revolutionizing plant biochemistry. Curr. Opin. Plant Biol. 15: 338-344.

84.   Bell, S.M., L.D. Burgoon and R.L. Last. 2012. MIPHENO: Data normalization for high throughput metabolite analysis. BMC Bioinformatics. BMC Bioinformatics 2012, 13:10. doi:10.1186/1471-2105-13-10.

85. Gonzales-Vigil, E., D.E. Hufnagel, J. Kim, R.L. Last and C. S. Barry. 2012. Evolution of TPS20-related terpene synthases influences chemical diversity in the glandular trichomes of the wild tomato relative Solanum habrochaites. Plant J. 71: 921–935. DOI: 10.1111/j.1365-313X.2012.05040.x

86. Milo, R. and R.L. Last. 2012. Achieving diversity in the face of constraints - lessons from metabolism. Science. 336:1663-1667. DOI: 10.1126/science.1217665

87. Schilmiller, A.L., A.L. Charbonneau and R.L. Last. 2012. Identification of a BAHD acetyltransferase that produces protective acyl sugars in tomato trichomes. Proc. Natl. Acad. U.S.A. 109:16377-16382. doi: 10.1073/pnas.1207906109 .

88. Kim, J., K. Kang, E. Gonzales-Vigil, F. Shi, C.S. Barry, A.D. Jones and R.L. Last. 2012. Striking natural diversity in glandular trichome acylsugar composition is shaped by variation at the ###i locus in the wild tomato Solanum habrochaites. Plant Physiol. 160:1854-1870. ###a href="doi:10.1104/pp.112.204735" class="redactor-linkify-object">http://www.ncbi.nlm.nih.gov/pm...

89. Savage, L.J., K. Imre, D.A. Hall, and R.L. Last. 2013. Analysis of essential Arabidopsis nuclear genes encoding plastid-targeted proteins. PLoS ONE 8(9): e73291. doi:10.1371/journal.pone.0073291.

90. Kim, J., Y. Matsuba, J. Ning, A.L. Schilmiller, D. Hammar, A.D. Jones, E. Pichersky and R.L. Last. 2014. Analysis of natural and induced variation in tomato glandular trichome flavonoids identifies a gene not present in the reference genome. Plant Cell 26: 3272-3285. doi: http://dx.doi.org/10.1105/tpc.114.129460

91. Fristedt, R., A. Herdean, C.E. Blaby-Haas, F. Mamedov, S.S. Merchant, R.L. Last and B. Lundin. 2014. PSB33, a protein conserved in the plastid lineage, is associated with the chloroplast thylakoid membrane and provides stability to Photosystem II supercomplexes in Arabidopsis. Plant Physiol. 167:481-492.  http://dx.doi.org/10.1104/pp.114.253336

92. Schilmiller, A.L., G.D. Moghe, P. Fan, B. Ghosh, J. Ning, A.D. Jones, R.L. Last. 2015. Functionally divergent alleles and duplicated loci encoding an acyltransferase contribute to acylsugar metabolite diversity in Solanum trichomes. Plant Cell 27:1002-1017. doi: 10.1105/tpc.15.00087

93. Liu, J. and R.L. Last. 2015. A land plant-specific thylakoid membrane protein contributes to photosystem II maintenance in ###i. Plant J.  82: 731–743. ###a href="http://onlinelibrary.wiley.com/doi/10.1111/tpj.12845/full">doi: 10.1111/tpj.12845

94. Peng, C., S. Uygun, S.-H. Shiu and R.L. Last. 2015. The impact of the branched-chain ketoacid dehydrogenase complex on amino acid homeostasis in Arabidopsis. Plant Physiol.  169: 1807-1820.   doi:10.1104/pp.15.00461

95. Ning, J., G.D. Moghe, B. Leong, J. Kim, I. Ofner, Z. Wang, C. Adams, A.D. Jones, D. Zamir and R.L. Last. 2015. A feedback insensitive isopropylmalate synthase affects acylsugar composition in cultivated and wild tomato. Plant Physiol. 169: 1821-1835.  doi:10.1104/pp.15.00474

96. Moghe, G.D. and R.L. Last. 2015. Something old, something new: Conserved enzymes and the evolution of novelty in plant specialized metabolism. Plant Physiol. 169: 1512-1523. doi:10.1104/pp.15.00994

97. Liu, J. and R.L. Last. 2015. MPH1 is a thylakoid membrane protein involved in protecting photosystem II from photodamage in land plants. Plant Signaling and Behavior. 10 (10):e1076602. doi:10.1080/15592324.2015.1076602

98. Coruzzi, G.M., R.L. Last, N. Dudareva, and N. Amrhein. 2015. Amino Acids. In: Biochemistry and Molecular Biology of Plants. R.B. Buchanan, W. Gruissem and R. Jones, eds. John Wiley & Sons, Ltd. pp. 289-336.

99. Schilmiller, A.L., K. Gilagon, B. Ghosh, A.D. Jones and R.L. Last. 2016. Acylsugar acylhydrolases: carboxylesterase catalyzed hydrolysis of acylsugars in tomato trichomes. Plant Physiol. 170:1331-1344 http://dx.doi.org/10.1104/pp.15.01348

100.  Fan, P., A.M. Miller, A.L. Schilmiller, X. Liu, I. Ofner, A.D. Jones, D. Zamir and R.L. Last. 2016. In vitro reconstruction and analysis of evolutionary variation of the tomato acylsucrose metabolic network. Proc. Natl. Acad. USA, 113 (2) E239-E248. doi/10.1073/pnas.1517930113.

101.  Fan, P., G.D. Moghe and R.L. Last. 2016. Comparative biochemistry and in vitro pathway reconstruction as powerful partners in studies of metabolic diversity. Methods in Enzymology, Volume 576, 2016, Pages 1–17. doi:10.1016/bs.mie.2016.02.023.

102.  Smeda, J.R., A.L. Schilmiller, R.L. Last and M. Mutschler. 2016. Introgression of acylsugar chemistry QTL modifies the composition and structure of acylsugars produced by high-accumulating tomato lines. Molec. Breeding, Mol Breeding (2016) 36: 160. doi:10.1007/s11032-016-0584-6.

103.  Uygun, S., C. Peng, M. Lehti-Shiu, R.L. Last, S.-H. Shiu, 2016. Utility and limitations of using gene expression data to identify functional associations. PLoS Comput Biol 12(12): e1005244. doi:10.1371/journal.pcbi.1005244.

104.  Xing, A. and R.L. Last. 2017. A regulatory hierarchy of the Arabidopsis branched-chain amino acid metabolic network. Plant Cell 29: 1480–1499. 10.1105/tpc.17.00186.

105.  Leong, B. and R.L. Last. 2017. Promiscuity, impersonation and accommodation: evolution of plant specialized metabolism. Curr. Opin. Struct. Biol., Volume 47, December 2017, Pages 105–112 https://doi.org/10.1016/j.sbi.2017.07.005.

106.  Liu, J. and R.L. Last. 2017. A chloroplast thylakoid lumen protein is required for proper photosynthetic acclimation of plants under fluctuating light environments. Proc. Natl. Acad. Sci. USA, 114(38):E8110-E8117, doi: 10.1073/pnas.1712206114.

107.  Moghe, G.D., B.J. Leong, S. Hurney, A.D. Jones and R.L. Last. 2017. Evolutionary routes to biochemical innovation revealed by integrative analysis of a plant defense related specialized metabolic pathway. eLife 2017;6:e28468 doi: https://doi.org/10.7554/eLife.28468.

108.  Fan, P., A.M. Miller, X. Liu, A.D. Jones and R.L. Last. 2017. Evolution of a flipped pathway creates metabolic innovation in tomato trichomes through BAHD enzyme promiscuity. Nat. Comm. 8, Article number: 2080. DOI: 10.1038/s41467-017-02045-7

109.  Xu, H., G.D. Moghe, K. Wiegert-Rininger, A.L. Schilmiller, C.S. Barry, R.L. Last and E. Pichersky. 2018. Identification of dehydrogenases involved in the biosynthesis of the monoterpene moiety of pyrethrin. Plant Physiol. Vol. 176: 524–537 DOI: https://doi.org/10.1104/pp.17.01330

110.  Xu, H., D. Lybrand, S. Bennewitz, A. Tissier, R.L. Last and E. Pichersky. 2018. Production of trans-chrysanthemic acid, the monoterpene acid moiety of natural pyrethrin insecticides, in tomato fruit. Metab. Eng. 47:271-278 https://doi.org/10.1016/j.ymben.2018.04.004.

111.  Moore, B.M., P. Wang, P. Fan, B. Leong, C.A. Schenck, J.P. Lloyd, M. Lehti-Shiu, R.L. Last, E. Pichersky, S.-H. Shiu. 2019. Robust predictions of specialized metabolism genes through machine learning, in press at Proc. Natl. Acad. Sci, USA 116:2344-2353. https://www.pnas.org/content/116/6/2344.short

112.  Leong, B.J., D. Lybrand, Y.-R. Lou, P. Fan, A.L. Schilmiller, R.L. Last. 2019. Evolution of metabolic novelty: a trichome-expressed invertase creates specialized metabolic diversity in wild tomato. Sci. Advances 5 (4), eaaw3754 https://advances.sciencemag.org/content/5/4/eaaw3754.abstract

113.  Fan, P., B.J. Leong, R.L. Last. 2019. Tip of the trichome: evolution of acylsugar metabolic diversity in Solanaceae. Curr. Opin. Plant Biol. 49:8-16. https://www.sciencedirect.com/science/article/pii/S1369526618300906?via%3Dihub

114.          Last, R.L. 2019. Put on your sunscreen: the birth of Arabidopsis stress genetics. Plant Cell. http://www.plantcell.org/content/31/7/1406.long.

115.      Liu, J., Y. Lu, W. Hua and R.L. Last. 2019. A new light on photosystem II maintenance in oxygenic photosynthesis. Front. Plant Sci. 10:975. doi: 10.3389/fpls.2019.00975

Volunteer Activities

President , American Society of Plant Biologists, October 2018-September 2019

Program Committee (organizers of the annual meeting), American Society of Plant Biologists, 2015-2018

co-Organizer, Plant Biotechnology for Health and Sustainability Symposium, Michigan State University, Fall semester 2013-. Faculty organizer for the associated Career Symposia, 2013-.

Associate Editor, Science Advances, 2014-

External Advisory Board, BioDiscovery Institute, University of North Texas, https://research.unt.edu/institutes/bdi, 2015-.

External Science Advisory Board, Missouri EPSCoR Program ‘Missouri Transect: Climate, Plant and Community’ (http://missouriepscor.org/). 2014-


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