Bio

Dr. Sunil Dutta Purohit was initiated in research at the Department of Botany, University of Jodhpur, by Professor H.C. Arya in July, 1977. He was awarded a Junior Research Fellowship of the University Grants Commission, New Delhi, funded research project entitled "Nature of Autonomous Growths in Plants Induced by Various Agencies" in February 1978. Dr. Purohit analysed abnormally grown tissues to find out the change in activities of enzymes like IAA- oxidase, peroxidase, polyphenol oxidase, ascorbic acid oxidase, alpha-amylase, and catalase and various metabolites like total and o-dihydroxy phenols, proteins, aromatic amino acids, carbohydrates, auxins, sulfhydryls and ascorbic acid as compared to normal tissue. He used various experimental plant materials like Zizyphus mauritiana stem galls, Prosopis cineraria stem and rachial galls, Giesekia pharnacoides leaf galls, Cordia myxa leaf galls, Ipomoea pentaphylla stem galls, and phyllody of Sesamum indicum induced by mycoplasma-like organisms (MLO) for the analyses. Dr. Purohit was awarded a Senior Research Fellowship of Council of Scientific and Industrial Research, New Delhi in February 1980. He submitted his Ph.D. thesis to the University of Jodhpur, Jodhpur in October 1980 on the problem entitled "Studies on Deranged Physiology of Sesamum phyllody induced by Mycoplasma-like Organisms (MLO)" and awarded degree in the year 1981.

            First research paper of Dr. Purohit entitled, "Light Microscopic Detection of Mycoplasma-like Organisms in Sesamum Phyllody" appeared in Current Science in the year 1978. He, subsequently published more than 80 research papers in the Journals like Plant Cell Reports (USA), Plant Cell Tissue and Organ Culture (The Netherlands), Plant Science (USA), Journal of Sustainable Forestry (USA), In Vitro Cell and Developmental Biology-Plant, Cytologia (Japan), European Journal of Horticultural Sciences (Germany), Biologia Plantarum (Czech Republic), Acta Physiologiae Plantarum (Germany), Scientia Horticulturae (The Netherlands), Journal of Asian and Australasian Plant Science and Biotechnology (UK), Medicinal and Aromatic Plant Science and Biotechnology (UK), International Journal of Plant and Developmental Biotechnology (UK), Indian Journal of Experimental Biology, Indian Journal of Biotechnology, Current Science, Science and Culture, Indian Phytopathology and Journal of Indian National Science Academy. He also availed of the opportunity to present his research results in various National and International symposia and conferences in the country and abroad.

            Dr. Purohit was appointed Research Associate in the Department of Science & Technology funded research project on "Rapid Multiplication of Economically Important Plants of Arid-zone through Tissue Culture, Differentiation and Establishment" in February 1981. He was successful in multiplication of desert plants like Zizyphus, Prosopis, Eucalyptus and few others through tissue culture.

            In July 1981, on the travel grant received from Department of Science and Technology, Government of India, Dr. Purohit visited Oxford, England to attend Joint Phytopathological Meetings organised by Association of Applied Biologists, Federation of British Plant Pathologists and International Society of Plant Pathology where he presented his research findings. He also visited few other places in England like Rothamsted Agricultural Experimentation Research Station, Herpenden, John Innes Institute, Norwich, Commonwealth Mycological Institute and Royal Botanical Gardens, Kew, London and National Virology Institute, Oxford.

            Dr. Purohit joined Department of Botany, Mohanlal Sukhadia University, Udaipur in July 1984 as Assistant Professor. He organised a well equipped Plant Biotechnology Laboratory to carry out research in the field of his interest. The major research support amounting to approximately Rs.10 million was received from University Grants Commission (UGC), Department of Biotechnology (DBT) Department of Science and Technology (DST), Council of Scientific and Industrial Research (CSIR), New Delhi; State Department of Science and Technology, Jaipur and Indian Council of Forestry Research and Education (ICFRE), Dehradun over a period of 15 years.

            The main focus of his research remained on micropropagation of plants of medicinal, forestry and horticultural importance. Under different research programmes, he has been able to develop micropropagation protocols for Feronia limonia, Wrightia tomentosa, W. tinctoria, Boswellia serrata, Sterculia urens, Acacia catechu, A. leucophloea (all woody trees),  Achras sapota, Annona squamosa (fruit trees), Celastrus paniculatus, Vitex negundo and Chlorophytum borivilianum (medicinal herb). Scaling-up production has been taken-up in the case of Feronia limonia, Wrightia tinctoria, W. tomentosa, B. serrata and C. borivilianum. Small-scale field performance of micropropagated plants has been successfully demonstrated in many species.

            Dr. Purohit was selected as Associate Professor of Botany in July 1997 in the same department. He was instrumental in development of Post-Graduate Courses in Biotechnology in this university which was introduced from the session 1997-98.  

Department of Science and Technology, Government of India, awarded SERC Visiting Fellowship to him to work with Professor A.K. Tyagi, Head, Department of Plant Molecular Biology, Co-ordinator, Centre for Plant Molecular Biology and Co-ordinator (India), International Rice Genome Project, University of Delhi, South Campus, New Delhi. Dr. Purohit, during his stay in the Department, learned basics of Practical Molecular Biology. He engaged himself in research on genetic transformation of Feronia limonia, a fruit tree species and chloroplast based Restriction Fragment Length Polymorphism (RFLP) to identify clones of Aegle marmelos and Feronia limonia. Dr. Purohit used Agrobacterium and Biolistics-mediated gene transfer system. He has learnt methods for isolation of genomic DNA of plants and hybridization techniques for molecular characterisation of variability among tissue culture clones, based on RFLP pattern in chloroplast genome. Dr. Purohit has recently taken up studies on molecular characterization of diversity among wild and tissue culture raised populations of Feronia limonia using ISSR primers and PCR amplification technique. Work on over-expression of rice chitinase gene in groundnut against Tikka diseases through Agrobacterium mediated gene transfer system has also been undertaken. Studies on molecular and cytological characterization have been carried out in natural and tissue culture raised plants of Chlorophytum borivilianum. Under a collaborative research program with Munich Technical University, Germany ,incorporation studies have been carried out using U13 C labelled glucose in tissue culture raised Boswellia serrata to determine the  pathway of Boswellic acid biosynthesis through NMR analysis. Phytochemical profiling has also been carried out in different accessions of Celastrus paniculatus in collaboration with PERD Centre, Ahmedabad (Dr. Mrs. M. Rajani) using HP-TLC and other analytical methods. Dr. Purohit has opened up a new area of investigation by demonstrating that improvement in growth and hardening of tissue culture plants  is possible by growing plants under CO2 enriched environments.

Dr. Purohit was appointed Course Director, Department of Biotechnology, in this University, in August 2004, the position which he is holding till date.

Dr. Purohit has undergone training on Microarray Based Genome Analysis under the guidance of Professor David Galbraith at the Department of Plant Sciences, Arizona State University,Tucson, Arizona, USA from January 8-13, 2006.

Dr. Purohit was promoted as professor, Department of Botany on 17###sup/sup### May, 2007. He has sanctioned a UGC project to study the effect of liquid culture system on growth, multiplication, physiology and morphology of some medicinally important plants. He also contemplates to study the photosynthetic efficiency of such plants. The Department of Science and Technology, Government of India, New Delhi has sanctioned a research project to study natural diversity in Chlorophytum borivilianum using molecular markers. He has been nominated on the task force constituted by the Government of Rajasthan for policy formulation on “Agricultural Education and Research Prioritization” in the State. Recently, he has been nominated by ICFRE as a Member of Research Advisory Group, Arid Forestry Research Institute, Jodhpur. Dr. Purohit delivered a plenary lecture during the International Medicinal and Aromatic Plant Symposium organized by Shiraz University and International Society for Horticultural Sciences, Shiraz, Iran in June, 2010. He was invited to deliver a guest lecture at an International Symposium on Medicinal Plants held at Montreal, Canada in the year 2013. He also visited Harvard University and MIT, USA in connection with his research in the same year. He was appointed Member of Advisory Committee for International Society for Phytocosmetic Sciences and Natural Products and was placed on the Editorial Board of an International Journal of Phytocosmetics and Natural Products, Boston, USA.

B.        Major areas of scientific enquiry

1.               Development of Field Tested micropropagation protocols for large-scale production of plants of forestry, horticultural and medicinal importance.

2.               Development of regeneration protocols via adventitious shoot bud differentiation and somatic embryogenesis.

3.               Studies on the role of liquid culture system for growth and multiplication of plants in vitro.

4.               Studies on CO2 enrichment during in vitro growth and development of plants.

5.               Studies on morphophysiological and biochemical characteristics of tissue culture raised plantlets.

6.               Studies on culture vessel, vessel ventilation and gelling agent on micropropagation of plants.

7.               Studies on use of VAM and other fungi for biopriming.

8.               Encapsulation of shoot buds, their long term storage, and studies on their regrowth in vitro.

9.               Molecular characterization of diversity in plants being used for micropropagation using RAPD and ISSR markers.

10.            Screening of tissue culture raised plantlets for genetic fidelity using molecular (RAPD and ISSR) and cytological markers.

11.            Optimization of conditions for Agrobacterium-mediated gene transfer in plants with special reference to disease resistance.

12.            Analysis of mitotic complements and karyotype studies in tissue culture and naturally growing plants.

13.            Protein profiling of tissue culture and naturally growing plants using SDS-PAGE.

Career Status:
Faculty/Group Leader, Major Research University: Post-Tenure

Research Areas:
Molecular Biology, Botany, Applied Plant Biology

Publications

  • 1   Purohit, S.D., Ramawat, K.G. and Arya, H.C., 1978.  Light microscopic detection of Mycoplasma-like organisms (MLO) in Sesamum phyllody.  Curr. Sci. 47:866-867.

2.               Purohit, S.D., Ramawat, K.G., Shekhawat, N.S. and Arya, H.C., 1978.  Note on effect of witches broom infection on Tephrosia purpurea Pers. Leaves.  Legume Research 2: 49-50.

3.     Purohit, S.D., Shekhawat, N.S., Ramawat, K.G. and Arya, H.C., 1979.  Role of some oxidative enzyme and metabolites in Sesamum phyllody. Indian J. Exp. Biol. 17: 714-716.

4.               Purohit, S.D., Ramawat, K.G. and Arya, H.C., 1979.  Phenolics, peroxidase and phenolase as related to gall formation in arid zone plants.  Curr. Sci. 48: 714-716.

5.               Purohit, S.D., Ramawat, K.G. and Arya, H.C., 1979.  Polyphenols and some oxidative enzymes in rust infected leaves of Cyperus rotundusIndian Phytopath.  32: 255-259.

6.               Ramawat, K.G., Purohit, S.D. and Arya, H.C., 1979.  Altered state of oxidizing enzymes and phenolics of Cordia galls. Trans. Isdt and Ucds (India) 4: 38-41.

7.               Purohit, S.D., Shekhawat, N.S., Tandon, P. and Arya, H.C., 1980.  Hormonal profiles in some insect and mite induced plant galls.  Proc. Indian Nat. Sci. Acad. B46 (6): 882-900.

8.               Purohit, S.D., Ramawat, K.G. and Arya, H.C., 1980.  Metabolic characteristics at enzymatic levels of Achyranthes leaves infected with white rust.  Indian J. Exp. Biol. 18: 98-99.

9.               Ramawat, K.G., Purohit, S.D. and Arya, H.C., 1980.  Phenolics and oxidative enzymes in normal and gall tissues of Giesekia. Sci. and Cult. 46: 11-12.

10.            Purohit, S.D., Purohit, S., Shekhawat, N.S. and Arya, H.C., 1980.  Some biochemical changes induced by Curvularia prasadii in Sesamum indicum leaves.  Comp. Physiol. Ecol. 5: 238-241.

11.            Purohit, S.D., Shekhawat, N.S. and Arya, H.C., 1980.  Hyperauxiny as related to Sesamum phyllody induced by mycoplasma like organisms (MLO).  Indian J. Exp. Biol. 18: 1338-1340.

12.            Purohit, S.D., Shekhawat, N.S., Kant, U. and Arya, H.C., 1980.  In vitro studies on MLO infected Sesamum and Sclerospora graminicola infected pearl-millet.  Proceedings of Symposium on Plant Tissue Culture, Genetic manipulation and Somatic Hybridization of Plant Cells, BARC, Trombay, Bombay (India) Feb. 27-29, pp. 163-168.

13.            Arya, H.C., Shekhawat, N.S. and Purohit, S.D., 1981.  Accumulation of aromatic compounds as related to abnormal growth in plants.  J. Indian Bot. Soc. 60: 247-51.

14.            Shekhawat, N.S., Purohit, S.D. and Arya, H.C., 1983.  Relative role of oospores and infected seed in green-ear disease incidence. Indian J. Mycology and Plant Pathology. 13: 17-20.

15.            Purohit, S.D., Shekhawat, N.S. and Arya, H.C., 1983.  Ascorbic acid metabolism in phyllody of Sesame induced by mycoplasma-like organism.  International Journal of Tropical Plant Diseases 1: 107-110.

16.            Purohit, S.D., Purohit, S., Rathore, T.S., Goyal, Y., Vaishnava, G.R., Surana, N.M. and Arya, H.C., 1983.  Clonal propagation of some trees species through tissue culture.  Proc. Natl. Symp. Adv. Front. Pl. Sci. pp. 129-130.

17.            Shekhawat, N.S., Galston, A.W., Arya, I.D., Purohit, S.D. and Arya, H.C., 1983.  Isolation, culture and regeneration of food legume protoplasts.  Proc. Natl. Symp. Adv. Front. Pl. Sci. pp. 159-160.

18.            Dhir, S.K., Purohit, S.D., Shekhawat, N.S., Purohit, S. and Arya, H.C., 1983.  Energy source plant: Calotropis procera.  Proc. Natl. Symp. Adv. Front. Pl. Sci. 147-148.

19.            Dhir, S.K., Shekhawat, N.S., Purohit, S.D. and Arya, H.C., 1984.  Development of laticifer cells in callus cultures of Calotropis procera (Ait) R. Br. Plant Cell Reports 3: 206-209.

20.            Shekhawat, N.S., Purohit, S.D. and Arya, H.C., 1984.  Changes in isoenzymes of peroxidase in green-ear of pearl-millet.  Curr. Sci.  53: 1157-1158.

21.            Arya, H.C., Shekhawat, N.S., and Purohit, S.D., 1990. Phytopathogen interaction, physiology and control aspects in vivo and In vitro state.  Proc. Natl. Symp. Adv. Pl. Sci.: Current Status & Emerging Challanges pp 441-450.

22.            Purohit, S.D. and Arya, H.C., 1990.  Efficacy of certain antibiotics against Sesamum Phyllody caused by mycoplasma-like organisms (MLO). Oikoassay 7: 1-4.

23.            Purohit, S.D., 1990. Polyamine Metabolism in Plants. Proc. Natl. Symp. Adv. Plant Sci.: Current Status and Emerging Challanges pp. 181-191.

24.            Purohit, S.D., Ramawat, K.G. and Arya, H.C., 1990.  IAA-oxidase, Peroxidase, Polyphenol oxidase and Phenolics in Ipomoea pentaphylla gall tissues.  Oikoassay 7: 29-32.

25.            Purohit, S.D. and Sukhwal, R., 1991. Induction and Maintenance of Embryogenic callus cultures of Apluda mutica L. Acta Ecologica 13: 137-140.

26.            Purohit, S.D. and Tak, K., 1992. In vitro propagation of an adult tree Feronia limonia L.  through axillary branching.  Indian J. Exp. Biol. 30: 377-379.

27.            Sukhwal, R., Kukda, G. and Purohit, S.D., 1992.  Biochemical status of Apluda mutica L. infected with Puccinia apludaeBionature 12: 29-31.

28.            Purohit, S.D., Kukda, G. and Sukhwal, R., 1992. Plant Regeneration in Apluda mutica L. through somatic embryogenesis. Indian J. Exp. Biol. 30: 1154-1157.

29.            Purohit, S.D., Tak, K., Kukda, G., Dave, A. and Sharma, P., 1992.  Propagation of some tree species of Aravallis in Rajasthan through tissue culture biotechnology Proc. Workshop on Tree Improvement and Provenance Research April 20-21, State Forest Department, Jaipur pp. 238-249.

30.            Purohit, S.D. and Kukda, G. 1993. Somatic embryogenesis and plantlet regeneration in Themeda quadrivalvis. Oikoassay 10: 3-4.

31.            Sukhwal, R. and Purohit, S.D. 1994. Effect of polyamines and their biosynthetic inhibitors on the growth of Helminthosporium turcicum. Bionature 14:47-50.

32.            Purohit, S.D., Dave, A. and Kukda, G. 1994. Micropropagation of Safed Musli (Chlorophytum borivilianum), a rare Indian medicinal herb. Plant Cell Tissue and Organ Culture 39: 93-96.

33.            Purohit, S.D. and Kukda, G. 1994. In vitro propagation of Wrightia tinctoria. Biologia Plantarum 36: 519-526.

34.            Purohit, S.D., Kukda, G., Sharma, P. and Tak, K. 1994. In Vitro propagation of an adult tree - Wrightia tomentosa through enhanced axillary branching. Plant Science 103: 67-72.

35.            Purohit, S.D., Kukda, G., Tak, K and Sharma, P., 1994. Micropropagation of two Wrightia species through enhanced axillary branching in vitro. In Frontiers of Plant Sciences, (Editor: I.A. Khan) Ukaaz Publications, Hyderabad. pp. 269-272.

36.            Purohit, S.D., Dave, A. and Tiagi, Y.D. 1994. Chlorophytum borivilianum Sant. & Fern. (Liliaceae): an interesting species from the Aravallis in Rajasthan. Rheedea 4: 113-115.

37.            Purohit, S.D., Dave, A. and Kukda, G. 1994. Somatic Embryogenesis and Plantlet Regeneration in `Safed Musli' (Chlorophytum borivilianum) Indian Journal of Plant Genetic Resources 7 (1): 65-71.

38.            Purohit, S.D., Dave, A. and Kukda, G. 1994. In vitro conservation strategies for a rare medicinal herb-Safed Musli (Chlorophytum borivilianum Sant. et Fernand). Indian J. Pl. Genet. Resources 7(2): 201-204.

39.            Purohit, S.D. and Kukda, G. 1995. Embryogenesis and plant regeneration from tissue culture of Sehima nervosum (Rottl.) Stapf. Bionature 15: 27-30.

40.            Purohit, S.D., Tak, K. and Kukda, G. 1995. In vitro propagation of Boswellia serrata Roxb. Biologia Plantarum 37: 335-40.

41.            Purohit, S.D., and Dave, A. 1996. Micropropagation of Sterculia urens Roxb. - An Endangered Tree Species. Plant Cell Reports 15: 704-706.

42.            Purohit, S.D., Kukda, G. and Tak, K. 1996. Micropropagation of Wrightia tomentosa (Roxb.) Roem et Schult. Journal of Sustainable Forestry (USA) 3: 25-35.

43.            Purohit, S.D., Singhvi, A. and Tak, K. 1996. Biochemical characteristics of differentiating callus cultures of Feronia limonia (L.). Acta Physiologiae Plantarum, (Poland) 18:47-52.

44.            Purohit, S.D., and Kukda, G. 1996. Somatic embryogenesis and plantlet regeneration in Heteropogon contortus (L.) P. Beauv. Journal of Indian Botanical Society, 75:1-2.

45.            Purohit, S.D., Tak, K., Joshi, P. and Samar, D. 1998. Micropropagation of some woody tree species of Aravallis in Rajasthan In "Plant Tissue Culture and Molecular Biology-Applications and Prospects (Editor: Prof. P.S. Srivastava) Narosa Publishing House, N. Delhi pp. 84-125.

46.            Purohit, S.D. and Singhvi, A. (1998) Micropropagation of Achras sapota Through Enhanced Axillary Branching. Scientia Horticulturae 76: 219-229.

47.            Purohit, S.D., Singhvi, A. and Nagori, R. (1998). Micropropagation of Achras sapota: Optimization of Conditions for Rooting, Hardening and Acclimatization. In "Proceedings of the National Symposium on Emerging Trends in Plant Tissue Culture and Molecular Biology" (Ed. P.B. Kavikishore), Department of Genetics, Osmania University, Hyderabad. pp. 134-145.

48.            Purohit, S.D., Tak, K., Samar, D. and Nagori, R. (2000). Micropropagation and Field Performance of Some Tree Species of Aravallis. Encyclopedia Botanica (Editor: P.C. Trivedi), Pointer Publishers, Jaipur, India. pp 225-239.

49.            Purohit, S.D., (2000). Micropropagation Techniques in Plant Tissue Culture, From Research to Commercialization. A Decade of Support. Department Of Biotechnology, Ministry of Science and Technology, Government of India.

50.            Sukhwal, R. and Purohit S.D. (2000). Effect of Polyamines and Their Biosynthetic Inhibitors on the Growth of Helminthosporium species. In Vistas in Mycology and Plant Pathology (Ed. L. N. Nair), Commonwealth Publishers, New Delhi. pp 113-120.

51.            Dave, A. and Purohit, S.D. (2002). Factors Influencing In vitro Culture of ‘Safed Musli’ (Chlorophytum borivilianum) Oikoassay 15: 19-27.

52.            Purohit, S.D., Dave, A., Bilochi, G., and Joshi, N. (2003). Opportunities for Scaling-up Production and Commercial Cultivation of Micropropagated ‘Safed Musli’ (Chlorophytum borivilianum).In: Proc. Natl. Interactive Meet on Scope and Opportunities in Research and Business of Medicinal and Aromatic Plants (Eds.A.K. Mathur et al.) CIMAP, Lucknow, India.pp.387-398.

53.            Sukhwal, R. and Purohit, S. D. (2003) Accumulation of Phenolics and Changes in Activity of Oxidative Enzymes in Maize Infected with Helminthosporium species. Journal of Mycology and Plant Pathology 33: 41-44.

54.            Vyas, S. and Purohit, S.D. (2003). In vitro Shoot Growth and Multiplication in Wrightia tomentosa Under Controlled Carbon-di-oxide Environment. Plant Cell, Tissue and Organ Culture 75: 283-286.

55.            Dave, A., Bilochi, G. and Purohit, S. D. (2003). Scaling-up Production and Field Performance of Micropropagated Medicinal Herb ‘Safed Musli’ (Chlorophytum borivilianum). In Vitro Cell Dev. Biol. (Plant). 39: 419-425.

56.            Purohit, S. D., Joshi, P., Tak, K. and Nagori, R. (2004). Development of High Efficiency Micropropagation Protocol For an Adult Tree – Wrightia tomentosa. In: “Plant Biotechnolohy and Molecular Markers “(Eds. Prof. P. S. Srivastava et al.) Anamaya Publishers, New Delhi, pp.217-227.

57.            Purohit, S. D. and Nagori, R. (2004). In vitro Plantlet Regeneration in Annona squamosa through Direct Shoot Bud Differentiation on Hypocotyl Segments. Scientia Horticulturae. 99: 89-98.

58.            Purohit, S. D. and Kukda, G. (2004) Micropropagation of an Adult Tree - Wrightia tinctoria Indian Journal of Biotechnology 3: 216-220.

59.            Purohit, S. D., Singhvi, A., Nagori, R. (2004). In vitro Direct Shoot Bud Differentiation from Leaf Segments of Achras sapota. Biologia Plantarum 48: 109-112.

60.            Dave A., Joshi, N. and Purohit S.D. (2004) In vitro propagation of Chlorophytum borivilianum using Encapsulated Shoot Buds. European J. Hort. Sci. 69: 37-42.

61.            Nagori, R and Purohit, S.D. (2004) Evidence for Somatic Embryogenesis in Annona squamosa Bionature 24: 1-6.

62.            Nandwani, D. Purohit S.D. Jain, S. M. and Ramawat K.G. (2004) Propagation Techniques in Woody Plants with Special Reference to Arid and Semi-Arid Zone Tree – Problems, Perseverance and Prospects In: “Tree Improvement and Biotechnology (Eds. P. Shanmugavel and Rev. Fr.S. Ignacimuthu, S.J.) Pointer Publishers, Jaipur, India. Pp.16-52.

63.            Dave, N. and Purohit, S.D. (2004) In vitro Shoot Growth and Multiplication in Achras sapota under Controlled Carbon Dioxide Environment. Biologia Plantarum 621-624.

64.            Vyas, S. Joshi, N., Tak. K. and Purohit, S.D. (2005) In vitro Adventitious Shoot Bud Differentiation and Plantlet Regeneration in Feronia limonia L.(Swingle). In Vitro Cell. Dev. Biol.- Plant 41: 296-309.

65.            Joshi, H., Chaudhary, B.L. and Purohit, S. D. (2005) Effect of Plant Growth Regulators on in vitro shoot multiplication in Nicotiana tabacum L. Indian J. Applied and Pure Biol. 20: 145-148.

66.            Vyas, S. and Purohit, S.D. (2005) Effect of Controlled Carbon Dioxide on In vitro Shoot Multiplication in Feronia limonia (L.) Swingle. Acta Physiologiae Plantarum 28:417-423.

67.            Joshi, P., Joshi, N. and Purohit, S.D. (2006) Stomatal Characteristics during Micropropagation of Wrightia tomentosa.  Biologia Plantarum 50: 275-278.

68.            Rao, M.S. and Purohit, S.D. (2006) In vitro Shoot Bud Differentiation and plantlet Regeneration in Celastrus paniculatus Willd. Biologia Plantarum 50: 501-506.

69.            Joshi, N., Kumar, A., Ramarao, S and Purohit, S.D. (2006) Cytological characterization of a rare medicinal herb ‘Safed Musli’ (Chlorophytum borivilianum Saint et Fernand.) Cytologia 71: 153-159.

70.            Trivedi, R. and Purohit, S. D. (2007) Factors affecting in vitro rooting in tissue culture derived shoots of groundnut (Arachis hypogaea L.) var. JL-24. Oikoassay 19: 1-16.

71.            Purohit, S. D., Singhvi, A. Vyas S. and Nagori R. (2006). Polyamines stimulated Shoot Bud Proliferation and Multiplication in Achras sapota Grown in Culture. Indian Journal of Biotechnology 6:85-90.

72.            Nagori, R., Vyas, S. and Purohit, S.D. (2007) Greenhouse growth and nursery performance of tissue culture raised plantlets of Achras sapota var. Cricket Ball. Indian Journal of Horticulture 64: 202-206.

73.            Purohit, S.D., Dave. A., Rathore, P., Vyas, S., Dave, N., Joshi, N. and Rao, M.S. (2007). Effect of Controlled Carbon Dioxide on In Vitro Shoot Multiplication in Some Plants of Horticultural, Forestry and Medicinal Importance. In: “Biotechnology: Plant Health Management” Professor H.N. Verma Commemoration Volume (Ed.Neeta Sharma and H.B. Singh), International Book Distributing Co., Lucknow. pp. 741-759.

74.            Purohit, S.D. Raghuvanshi, S. and Tyagi, A.K. (2007) Biolostic-mediated DNA Delivery and Transient Expression of GUS Gene in Hypocotyls of a Fruit Tree- Feronia limonia Indian Journal of Biotechnology 6:504-507.

75.            Rathore, P., Suthar, R. and Purohit S.D. (2008) Micropropagation of Terminalia bellerica (Roxb.) from juvenile explants. Indian Journal of Biotechnology 7:246-249.

76.            Nagori, R., Vyas, S., Tak, K., Singhvi, A. and Purohit, S.D. (2008) In vitro adventitious shoot bud differentiation and plantlet regeneration in some fruit plants. Proceeding NSPB-2006, Dehradun, 297-317.

77.            Mathur, P. Rao M.S. and Purohit, S.D. (2008). Genetic stability in micro-clones of Celastrus paniculatus Willd. derived from different explant sources as revealed by RAPD profiles. Asian and Australasian Journal of Plant Science and Biotechnology 2(2): 99-91.

78.            Vyas, S., Gaur, A., Tyagi, A. and Purohit, S. D. (2008) Assessment of Genetic Diversity in Feronia limonia L. (Swingle) using ISSRs. Journal of Sustainable Forestry. 27(3): 328-342.

79.            Rao, M.S., and Purohit, S.D. (2008) In Vitro Growth and Multiplication of Celastrus paniculatus Willd. under Carbon Dioxide Enriched Environment Medicinal and Aromatic Plant Science and Biotechnology 2(2):  101-104. 

80.            Vyas, S., Rao, M.S., Suthar, R.K. and Purohit, S.D. (2008) Liquid Culture System Stimulates in Vitro Growth and Shoot Multiplication in Four Medicinally Important Plants Medicinal and Aromatic Plant Science and Biotechnology 2(2): 96-100.

81.            Joshi, N., Randhawa, G. J., Firke, P. K. and Purohit, S.D. (2008) RAPD Analysis of Diversity in ‘Safed Musli’ (Chlorophytum borivilianum Sant. et Fernand.), a Rare Indian Medicinal Herb. Medicinal and Aromatic Plant Science and Biotechnology 2(2):  86-89.

82.            Vyas, S., Nagori, R. and Purohit, S.D. (2008) Root Colonization and Growth Enhancement of Micropropagated Feronia limonia (L.) Swingle by Piriformospora indica – A Cultivable Root Endophyte International Journal of Plant Developmental Biology 2(2): 128-132.

83.            Suthar, R.K. and Purohit, S.D. (2008) Root Colonization and Improved Growth Performance of Micropropagated Terminalia bellerica Roxb. Plantlets Inoculated with Piriformospora indica during ex Vitro Acclimatization. International Journal of Plant Developmental Biology 2 (2): 133-136.

84.            Rao, M.S. and Purohit, S.D. (2008). In Vitro Regeneration in Celastrus paniculatus Willd. : Influence of Explant, Gelling Agent and Carbon Source. Proceedings of Indian National Science Academy 74:161-166.

85.            Mathur, P. and Purohit, S.D. (2008). Peroxidase and IAA oxidase activities during in vitro rooting of Chlorophytum borivilianum Sant. et Fernand. Oikoassay 21: 3-9.

86.            Trivedi, R and Purohit, S.D. (2009) Biology and Biotechnology of Groundnut (Arachis hypogaea L.)-A Review. Professor H.C. Arya Commemoration Volume (Editors: A. Kumar and N.S. Shekhawat) I.K. International Publishers, New Delhi. Pp. 315-331.

87.            Nagori, R., Rathore,P., Vyas, S., Joshi, N., Rao, M.S., Suthar, R., Mathur, P and Purohit, S.D. (2009) Liquid Culture System Stimulates In Vitro Growth and Shoot Multiplication in Some Medicinally Important Plant Species of Aravallis in Rajasthan. Professor H.C.Arya Commemoration Volume (Editors: A. Kumar and N.S. Shekhawat) I.K. International Publishers, New Delhi pp. 395-405.

88.  Vyas, S., Gaur, A., Tyagi, A.K. and Purohit, S.D. (2009) Molecular Characterization of Diversity in Feronia limonia using ISSR Markers. Professor H.C. Arya Commemoration Volume (Editors: A. Kumar and N.S. Shekhawat) I.K. International Publishers, New Delhi. Pp. 431-443.

89.            Joshi, N., Randhwa, G.J. and Purohit, S.D. (2009) Morphological and Molecular Characterization of a Rare Medicinal Herb ‘Safed Musli’ (Chlorophytum borivilianum Sant.Et Fernand) Professor H.C.Arya Commemoration Volume (Editors: A. Kumar and N.S. Shekhawat) I.K. International Publishers, New Delhi Pp. 519-530.

90.            Joshi, P. Trivedi, R. and Purohit, S.D. (2009) Micropropagation of Wrightia   tomentosa: Effect of gelling agents, carbon source and vessel type. Indian Journal of Biotechnology 8: 115-120.

91.            Habibi, N., Suthar, R.K. and Purohit, S.D. (2009) Role of PGRs and their inhibitors in induction and control of somatic embryogenesis in Themeda quadrivalvis.  Indian Journal of Experimental Biology 47: 198-203.

92.            Suthar, R.K. and Purohit, S.D. (2009). In vitro growth and multiplication of Terminalia bellerica Roxb. under carbon dioxide enriched environment. Indian Journal of Experimental Biology 47: 204-209.  

93.            Joshi, N., Dave, A., Vyas, S and Purohit, S.D. (2009) Effect of controlled carbon dioxide environment on in vitro growth and shoot multiplication in Chlorophytum borivilianam Sant. et Fernand. Indian Journal of Biotechnology 8:323-327.

94.            Sukhwal, R., Trivedi, R. and Purohit, S.D. (2009) In vitro screening of callus cultures of maize treated with toxins of Helminthosporium sps. The Journal of the Indian Botanical Society 88: 1-4.

95.            Purohit S.D. (2009). Green technology (biotechnology) for sustainable development: an appraisal. In:  Tiwari, R.C., Sharma, G.S. and Khandelwal S.K. (Eds.) Organic Farming for Sustainable Agriculture, Himanshu Publications, Udaipur.  pp. 1-8.

96.            Khan, K., Joshi, P. and Purohit, S.D. (2010) Stomatal Characteristics during Micropropagation of Wrightia Tomentosa. Acta Hort. (ISHS) 865: 187-191.

97.            Chittora, M., Suthar, R.K. and Purohit, S.D. (2010) Root Colonization and Improved Growth Performance of Micropropagated Terminalia Bellerica Roxb. Plantlets Inoculated With Piriformospora Indica During Ex Vitro Acclimatization. Acta Hort. (ISHS) 865: 193-198.

98.            Joshi, P., Vyas, S. and Purohit, S.D.  (2010) Photosynthetic Performance of Shoots of Feronia Limonia Grown In Vitro Under Carbon Dioxide Enriched Environment. Acta Hort. (ISHS) 865: 225-230.

99.            Suthar, R.K. and Purohit, S.D. (2010) Anatomy of Root Development in Tissue Culture Raised Terminalia Bellerica Roxb. and Its Significance in Hardening. ActaHort. (ISHS) 865: 123-127.

100.         Purohit, S.D. and Habibi, N. (2010) Effect of CO2 Enrichment on In Vitro     Growth, Hardening and Acclimatization during Micropropagation of Some Tree Species of Aravallis in Rajasthan. Acta Hort. (ISHS) 865: 149-162.

101.         Purohit, S.D. and Mathur, P. (2010). Assessment of clonal fidelity in micropropagated plants using genetic markers. In: “Current Trends in Plant Biology” P.C. Trivedi and S.K. Sopory (Eds.) pp 185-217, Aavishkar Publishers, Distributors, Jaipur, India.

102.         Purohit, S.D. and Suthar, R.K. (2010). Hardening and acclimatization of   micropropagated plants – success and bottlenecks. In: “Agricultural Biotechnology” P.C. Trivedi (Ed.) pp 155-171, Aavishkar Publishers, Distributors, Jaipur, India.

103.         Suthar R.K., Habibi, N.and Purohit, S.D. (2011) Influence of agar concentration and liquid medium on in vitro propagation of Boswellia serrata Roxb. Indian Journal of Experimental Biology 10: 224-227.  

104.         Nagori R. and Purohit S.D (2011). An improved method for DNA isolation from polyphenols rich leaf samples of Annona squamosa L. Journal of plant Biochemistry and Biotechnology 21: 140-143.

105.         Purohit, S.D., Teixeira da Silva J.A., Habibi, N. (2011) Current approaches for cheaper and better micropropagation technologies. International Journal of Plant Developmental Biology 5:1-36.

106.         Khan, K., Sharma, S.K., Ramarao, S and Purohit, S.D. (2011) Karyomorphological studies in Wrightia tomentosa and W. tinctoria: Multipurpose tree species of Aravallis in South-east Rajasthan Cytologia 76: 125-128.

107.         Sharma, P. and Purohit S.D. (2012) An improved method of DNA isolation from polysaccharides rich leaves of Boswellia serrata Roxb. Indian Journal of Biotechnology 11: 67-71.

108.         Chittora, M. and Purohit, S.D. (2012) Optimization of SDS-PAGE conditions and analysis of seed protein diversity in Abrus precatorious genotypes with different seed coat colours. International Journal of Life Sciences Biotechnology and Pharma Research Vol. 1 No. 2 268-277.

109.         Joshi, N. and Purohit, S.D. (2012) Optimization of factors influencing shoot multiplication during micropropagation of Chlorophytum borivilianum Sant et. Fernand Proceedings of the National Academy of Sciences, India Vol.82 (3) 421-429.

110.         Devpura, V. and Purohit S.D. (2012) Standardization of DNA extraction method and optimization of RAPD-PCR conditions in Anogeissus latifolia. Proceedings of the Indian National Science Academy Vol. 78(4): 621-627.

111.         Suthar R.K and Purohit, S.D. (2012) Bio Priming of Micropropagated Boswellia serrata Roxb. Plantlets –Role of endophytic root fungus Piriformospora indica Indian Journal of Biotechnology 11: 304-308.

112.         Joshi, P. and Purohit, S.D. (2012) Molecular screening and test of clonal fidelity in micro-clones of Feronia limonia (L.) swingle derived from different micropropagation pathways and explants sources Acta Hort. (ISHS ) Proc. 7th IS on in vitro Culture Horticulture Breeding.

113.          Mathur, P., Habibi, N., Chittora, M and Purohit, S.D. (2012) Molecular analysis of variability among three genotypes of Abrus precatorious L.with different seed coat colours using RAPD and ISSR markers. Indian Journal of Biotechnology (Revised).

114.         Suthar R.K and Purohit, S.D. (2012) Bio Priming of Micropropagated Boswellia serrata Roxb. Plantlets –Role of endophytic root fungus Piriformospora indica Indian Journal of Biotechnology 11: 304-308.

115.         Mathur, P., Habibi, N., Chittora, M and Purohit, S.D. (2012) Molecular analysis of variability among three genotypes of Abrus precatorious L.with different seed coat colours using RAPD and ISSR markers. Indian Journal of Biotechnology.

116.         Rao M; Nagori N, Sharma, P. Purohit S.D (2012) Shoot bud differentiation and plantlet regeneration from in vitro derived leaf explants of Celastrus paniculatus International Journal of Recent Scientific Res. 3:932-935.

117.         Purohit, S.D. and Habibi, N. (2014) Micropropagation of Some Medicinal Plants of Aravallis in Rajasthan-Cheaper and Better Options. Proc.International Symposium on Medicinal Plants and Natural Products. Acta Hort.1030, International Society for Horticultural Science. Pp109-117. (Quito, Ecuador).

118.         Nagori R. Sharma P., Habibi N. and Purohit S.D (2014). An efficient Genomic DNA extraction protocol for molecular analysis in Annona reticulta L.. Natl. Acad. Sci. Lett. DOI 10.1007/s40009-013-0213-4.

119.         Rao, M.S., Suthar, D., Purohit S.D. (2014) Scanning Electron Microscopic Study reveals stomatal malfunctioning in in vitro grown Celastrus paniculatus Wild. International Journal of Applied life Sciences and Engineering. 1(1): 44-50.

120.         Joshi, P., Suthar, D., Purohit, S.D. (2014) Effect of polymanies on in vitro growth, shoot multiplication and rooting in Wrightia tomentosa Roem et. Shult. International Journal of Recent Scientific Research. 5 (7): 1270 -1273.

121.         Babel, P., Devpura, V., Purohit S.D. (2014) Salicylic acid induced changes in growth and some biochemical characteristics in in vitro cultured shoots of Chlorophytum borivilianum Sant et. Fernand. International Journal of Recent Scientific Research. 5 (4): 774-779.

122.         Purohit, S.D., Sharma, P. and Nagori, R.N. (2015) Assessment of Molecular Diversity and in vitro clonal fidelity in some Indian Medicinal Plants. Proc.IS on MPNP Acta.Hort 1098.

123.         Joshi, N., Nagori, R.N., Sharma, P. and Purohit, S.D. (2015) Morphological and Phytochemical Characterization of Celastrus paniculatus germplasm from Aravallis of Rajasthan. Proc. IS on MPNP Acta.Hort 1098.

124.         Rao, M.S., Nagori, R.N., Sharma P. and Purohit,S.D. (2015) Studies on Natural Diversity and in vitro behaviour of a Rare Indian Medicinal Herb” Safed Musli” (Chlorophytum borivilianum). Proc. IS on MPNP Acta.Hort 1098.

125.         Vyas, S., Babel, P., Goswami, N. and Purohit, S.D. (2016) Evaluation of Leaf Ultra Structure and Chlorophyll contents during Micropropagation of Feronia limonia (L.) Swingle. International Journal of Advance Life Sciences 9:538-546.

126.         Sharma, P., Babel, P., Goswami, N. and Purohit S.D. (2018) Micropropagation of Acacia leucophloea (Roxb.) Willd. : A Multipurpose tree Legume. Proc. Natl. Sci. Acad. Section B 88: 1329-1335.

127.         Nagori, R., Sharma, P. and Purohit S.D. (2018). Molecular Characterization of Diversity in  Annona squamosa L.: A High Value Tropical Medicinal Plant. International Journal of Phytocosmetics and Natural Ingredients (USA) 5:1-13.

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